Oral branched-chain amino acid granules improve structure and function of human serum albumin in cirrhotic patients

• Published in: Journal of gastroenterology 2017-06, Vol.52 (6), p.754-765
• Main Authors: Setoyama, Hiroko, Tanaka, Motohiko, Nagumo, Kohei, Naoe, Hideaki, Watanabe, Takehisa, Yoshimaru, Youko, Tateyama, Masakuni, Sasaki, Masato, Watanabe, Hiroshi, Otagiri, Masaki, Maruyama, Toru, Sasaki, Yutaka
• Format: Article
• Language: English
• Subjects: Abdominal Surgery; Administration, Oral; Aged; Albumin; Amino acids; Amino Acids, Branched-Chain - administration & dosage; Amino Acids, Branched-Chain - metabolism; Analysis; Antioxidants; Antioxidants - administration & dosage; Antioxidants - metabolism; Biliary Tract; Branched chain amino acids; Case-Control Studies; Colorectal Surgery; Development and progression; Dietary Supplements; Disease Progression; Female; Free Radical Scavengers - administration & dosage; Free Radical Scavengers - metabolism; Gastroenterology; Hepatitis; Hepatitis, Chronic - blood; Hepatitis, Chronic - therapy; Hepatology; Human serum albumin; Humans; Liver; Liver cirrhosis; Liver Cirrhosis - blood; Liver Cirrhosis - therapy; Liver diseases; Male; Medical research; Medicine; Medicine & Public Health; Medicine, Experimental; Middle Aged; Original Article—Liver; Original —Liver, Pancreas, and Biliary Tract; Oxidation-Reduction; Oxidative stress; Pancreas; Serum Albumin, Human - metabolism; Structure-function relationships; Surgical Oncology
• ISSN: 0944-1174; 1435-5922
• DOI: 10.1007/s00535-016-1281-2

Background and aims The aim of this study was to evaluate structural and functional alterations of human serum albumin (HSA), with a special focus on the oxidized and reduced forms, in patients with chronic liver disease. We also investigated whether oral branched-chain amino acid (BCAA) supplementation could induce structural changes and improve the functions of HSA. Methods The proportion of reduced and oxidized HSA was determined in 16 healthy controls and in 20 chronic hepatitis and 100 cirrhotic patients with stable conditions. To evaluate the functional properties of HSA, this study focused on the antioxidant and binding functions. The radical scavenging activity and binding ability of purified HSA were measured in 68 participants. After BCAA administration for 6 months, 29 patients were evaluated for HSA structural changes, with 19 out of the 29 patients also analyzed for HSA functional changes. Results There was a significant decrease in the amounts of reduced HSA in conjunction with liver disease progression. Receiver operating characteristic curve analysis demonstrated that the levels of reduced HSA had high accuracy in determining disease progression. Functional alterations were strongly correlated to the levels of reduced HSA. BCAA supplementation led to substantial increases in the amount of reduced HSA. The altered HSA was able to scavenge significantly more radicals and restore the binding ability. Conclusion This study describes structural alterations and functional disturbances of HSA in patients with chronic liver disease, and indicates that the levels of reduced HSA might reflect disease progression and the functional properties of HSA. Moreover, oral BCAA supplementation increases the amount of reduced HSA, thereby leading to the restoration of HSA function in cirrhotic patients.