Skin‐Derived Precursors as a Source of Progenitors for Corneal Endothelial Regeneration

• Published in: Stem cells translational medicine 2017-03, Vol.6 (3), p.788-798
• Main Authors: Inagaki, Emi, Hatou, Shin, Higa, Kazunari, Yoshida, Satoru, Shibata, Shinsuke, Okano, Hideyuki, Tsubota, Kazuo, Shimmura, Shigeto
• Format: Article
• Language: English
• Subjects: Animals; Autografts; Blindness; Bone surgery; Cell culture; Cell Differentiation; Cell junctions; Cornea; Corneal endothelium; Corneal Transplantation; Dermis; Disease Models, Animal; Edema; Endothelial cells; Endothelial Cells - cytology; Endothelial Cells - metabolism; Endothelium; Endothelium, Corneal - cytology; Eye diseases; Graft rejection; Green Fluorescent Proteins - metabolism; Humans; Immunohistochemistry; Integrases - metabolism; Mice; Mice, Inbred C57BL; Mice, Transgenic; Na+/K+-exchanging ATPase; Neural crest; Neural stem cells; NIH 3T3 Cells; Polymerase chain reaction; Rabbits; Regeneration; Retinoic acid; Reverse transcription; Skin; Skin - cytology; Skin progenitors; Sodium-Potassium-Exchanging ATPase - metabolism; Spheroids, Cellular - cytology; Stem cell transplantation; Stem cells; Stem Cells - cytology; Tissue‐Specific Progenitor and Stem Cells; Transgenic animals; Translational s and Reviews; Western blotting
• ISSN: 2157-6564; 2157-6580
• DOI: 10.1002/sctm.16-0162

Corneal blindness is the fourth leading cause of blindness in the world. Current treatment is allogenic corneal transplantation, which is limited by shortage of donors and immunological rejection. Skin‐derived precursors (SKPs) are postnatal stem cells, which are self‐renewing, multipotent precursors that can be isolated and expanded from the dermis. Facial skin may therefore be an accessible autologous source of neural crest derived cells. SKPs were isolated from facial skin of Wnt1‐Cre/Floxed EGFP mouse. After inducing differentiation with medium containing retinoic acid and GSK 3‐β inhibitor, SKPs formed polygonal corneal endothelial‐like cells (sTECE). Expression of major corneal endothelial markers were confirmed by Reverse transcription polymerase chain reaction (RT‐PCR) and quantitative Real time polymerase chain reaction (qRT‐PCR). Western blots confirmed the expression of Na, K‐ATPase protein, the major functional marker of corneal endothelial cells. Immunohistochemistry revealed the expression of zonular occludens‐1 and Na, K‐ATPase in cell‐cell junctions. In vitro functional analysis of Na, K‐ATPase pump activity revealed that sTECE had significantly high pump function compared to SKPs or control 3T3 cells. Moreover, sTECE transplanted into a rabbit model of bullous keratopathy successfully maintained corneal thickness and transparency. Furthermore, we successfully induced corneal endothelial‐like cells from human SKPs, and showed that transplanted corneas also maintained corneal transparency and thickness. Our findings suggest that SKPs may be used as a source of autologous cells for the treatment of corneal endothelial disease. Stem Cells Translational Medicine 2017;6:788–798