Optimization of rhamnolipid production by Pseudomonas aeruginosa OG1 using waste frying oil and chicken feather peptone

In the present study, production of rhamnolipid biosurfactant by Pseudomonas aeruginosa OG1 was statistically optimized by response surface methodology. Box–Behnken design was applied to determine the optimal concentrations of 52, 9.2, and 4.5 g/L for carbon source (waste frying oil), nitrogen sourc...

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Bibliographic Details
Published in:3 Biotech 2017-06, Vol.7 (2), p.117-117, Article 117
Main Authors: Ozdal, Murat, Gurkok, Sumeyra, Ozdal, Ozlem Gur
Format: Article
Language:English
Subjects:
Agriculture
Bioinformatics
Biomaterials
biosurfactants
Biotechnology
Cancer Research
carbon
Carbon sources
Chemistry
Chemistry and Materials Science
chickens
Cultivation
Design optimization
Emulsification
emulsifying
experimental design
feathers
Frying
frying oil
nitrogen
Original
Original Article
Peptone
peptones
potassium dihydrogen phosphate
Pseudomonas aeruginosa
Response surface methodology
Rhamnolipids
Stem Cells
wastes
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Summary:In the present study, production of rhamnolipid biosurfactant by Pseudomonas aeruginosa OG1 was statistically optimized by response surface methodology. Box–Behnken design was applied to determine the optimal concentrations of 52, 9.2, and 4.5 g/L for carbon source (waste frying oil), nitrogen source (chicken feather peptone), and KH 2 PO 4 , respectively, in production medium. Under the optimized cultivation conditions, rhamnolipid production reached up to 13.31 g/L (with an emulsification activity of 80%), which is approximately twofold higher than the yield obtained from preliminary cultivations. Hence, rhamnolipid production, noteworthy in the literature, was achieved with the use of statistical optimization on inexpensive waste materials for the first time in the present study.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-017-0774-x