Repression of the yeast HO gene by the MATα2 and MATa1 homeodomain proteins

• Published in: Nucleic acids research 2004, Vol.32 (22), p.6469-6478
• Main Authors: Mathias, Jonathan R., Hanlon, Sean E., O'Flanagan, Ruadhan A., Sengupta, Anirvan M., Vershon, Andrew K.
• Format: Article
• Language: English
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/gkh985

The HO gene in Saccharomyces cerevisiae is regulated by a large and complex promoter that is similar to promoters in higher order eukaryotes. Within this promoter are 10 potential binding sites for the a1-α2 heterodimer, which represses HO and other haploid-specific genes in diploid yeast cells. We have determined that a1-α2 binds to these sites with differing affinity, and that while certain strong-affinity sites are crucial for repression of HO, some of the weak-affinity sites are dispensable. However, these weak-affinity a1-α2-binding sites are strongly conserved in related yeast species and have a role in maintaining repression upon the loss of strong-affinity sites. We found that these weak sites are sufficient for a1-α2 to partially repress HO and recruit the Tup1-Cyc8 (Tup1-Ssn6) co-repressor complex to the HO promoter. We demonstrate that the Swi5 activator protein is not bound to URS1 in diploid cells, suggesting that recruitment of the Tup1-Cyc8 complex by a1-α2 prevents DNA binding by activator proteins resulting in repression of HO.