Thymoquinone Defeats Diabetes-Induced Testicular Damage in Rats Targeting Antioxidant, Inflammatory and Aromatase Expression

• Published in: International journal of molecular sciences 2017-04, Vol.18 (5), p.919
• Main Authors: Atta, Mustafa S, Almadaly, Essam A, El-Far, Ali H, Saleh, Rasha M, Assar, Doaa H, Al Jaouni, Soad K, Mousa, Shaker A
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Antioxidants - metabolism; Aromatase; Aromatase - metabolism; Benzoquinones - pharmacology; Body Weight - drug effects; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - chemically induced; Diabetes Mellitus, Experimental - pathology; Glutathione - metabolism; Inflammation; Injection; Male; Males; Malondialdehyde; Malondialdehyde - metabolism; NF-kappa B - metabolism; Nitric oxide; Nitric Oxide - metabolism; Nitric Oxide Synthase Type II - metabolism; Nitric-oxide synthase; Oxidative Stress - drug effects; Protective Agents - pharmacology; Rats; Rats, Wistar; Reproductive system; Rodents; Sperm; Spermatogenesis; Spermatogenesis - drug effects; Streptozocin; Streptozocin - toxicity; Superoxide dismutase; Superoxide Dismutase - metabolism; Testes; Testis - drug effects; Testis - metabolism; Testis - pathology; Up-Regulation - drug effects
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms18050919

Antioxidants have valuable effects on the process of spermatogenesis, particularly with diabetes mellitus (DM). Therefore, the present study investigated the impact and the intracellular mechanisms by which thymoquinone (TQ) works against diabetes-induced testicular deteriorations in rats. Wistar male rats ( = 60) were randomly allocated into four groups; Control, Diabetic (streptozotocin (STZ)-treated rats where diabetes was induced by intraperitoneal injection of STZ, 65 mg/kg), Diabetic + TQ (diabetic rats treated with TQ (50 mg/kg) orally once daily), and TQ (non-diabetic rats treated with TQ) for 12 weeks. Results revealed that TQ significantly improved the sperm parameters with a reduction in nitric oxide (NO) and malondialdehyde (MDA) levels in testicular tissue. Also, it increased testicular reduced glutathione (GSH) levels and superoxide dismutase (SOD) activity. Interestingly, TQ induced downregulation of testicular inducible nitric oxide synthase (iNOS) and nuclear factor kappa-B (NF-κB) and significantly upregulated the aromatase protein expression levels in testicles in comparison with the diabetic rats. In conclusion, TQ treatment exerted a protective effect against reproductive dysfunction induced by diabetes not only through its powerful antioxidant and hypoglycemic effects but also through its downregulation of testicular iNOS and NF-κB along with upregulation of aromatase expression levels in diabetic rats.