Characterization of Copy Number Variation's Potential Role in Marek's Disease

Marek's Disease (MD) is a highly contagious pathogenic and oncogenic disease primarily affecting chickens. Chicken Lines 63 and 72, as well as their recombinant congenic strains (RCS) with varied susceptibility to MD, are ideal models to study the complex mechanisms of genetic resistance to MD....

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Published in:International journal of molecular sciences 2017-05, Vol.18 (5), p.1020
Main Authors: Xu, Lingyang, He, Yanghua, Ding, Yi, Sun, Guirong, Carrillo, Jose Adrian, Li, Yaokun, Ghaly, Mona M, Ma, Li, Zhang, Huanmin, Liu, George E, Song, Jiuzhou
Format: Article
Language:English
Subjects:
Animals
Chickens
Chickens - genetics
Copy number
Correlation
DNA Copy Number Variations
Gene Deletion
Gene expression
Genes
Genetic structure
Genetics
Genotyping
Inbreeding
Infectious diseases
Marek Disease - genetics
Marek's disease
Poultry
Segments
Single-nucleotide polymorphism
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Summary:Marek's Disease (MD) is a highly contagious pathogenic and oncogenic disease primarily affecting chickens. Chicken Lines 63 and 72, as well as their recombinant congenic strains (RCS) with varied susceptibility to MD, are ideal models to study the complex mechanisms of genetic resistance to MD. In this study, we investigated copy number variation (CNV) in these inbred chicken lines using the Affymetrix Axiom HD 600 K SNP genotyping array. We detected 393 CNV segments across all ten chicken lines, of which 12 CNVs were specifically identified in Line 72. We then assessed genetic structure based on CNV and observed markedly different patterns. Finally, we validated two deletion events in Line 72 and correlated them with genes expression using qPCR and RNA-seq, respectively. Our combined results indicated that these two CNV deletions were likely to contribute to MD susceptibility.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms18051020