Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells

To avoid morbidity and limited availability associated with autografts, synthetic calcium phosphate (CaP) ceramics were extensively developed and used as bone filling materials. Controlling their induced-inflammatory response nevertheless remained a major concern. Strontium-containing CaP ceramics w...

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Bibliographic Details
Published in:Materials 2016-12, Vol.9 (12), p.985-985
Main Authors: Braux, Julien, Velard, Frédéric, Guillaume, Christine, Jourdain, Marie-Laure, Gangloff, Sophie C, Jallot, Edouard, Nedelec, Jean-Marie, Laquerrière, Patrice, Laurent-Maquin, Dominique
Format: Article
Language:English
Subjects:
Arrays
Bioengineering
Biomaterials
Bones
Ceramics
Chemical Sciences
Communication systems
Human behavior
Human performance
Life Sciences
Material chemistry
Nuclear Experiment
Osteoblasts
Physics
Strontium
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Summary:To avoid morbidity and limited availability associated with autografts, synthetic calcium phosphate (CaP) ceramics were extensively developed and used as bone filling materials. Controlling their induced-inflammatory response nevertheless remained a major concern. Strontium-containing CaP ceramics were recently demonstrated for impacting cytokines' secretion pattern of human primary monocytes. The present study focuses on the ability of strontium-containing CaP to control the human primary bone cell production of two major inflammatory and pro-osteoclastogenic mediators, namely MCP-1 and Gro-α, in response to ceramics particles. This in vitro study was performed using human primary osteoblasts in which their response to ceramics was evaluated by PCR arrays, antibody arrays were used for screening and real-time PCR and ELISA for more focused analyses. Study of mRNA and protein expression highlights that human primary bone cells are able to produce these inflammatory mediators and reveal that the adjunction of CaP in the culture medium leads to their enhanced production. Importantly, the current work determines the down-regulating effect of strontium-substituted CaP on MCP-1 and Gro-α production. Our findings point out a new capability of strontium to modulate human primary bone cells' communication with the immune system.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma9120985