The Molecular Dialog between Flowering Plant Reproductive Partners Defined by SNP-Informed RNA-Sequencing

The molecular interactions between reproductive cells are critical for determining whether sexual reproduction between individuals results in fertilization and can result in barriers to interspecific hybridization. However, it is a challenge to define the complete molecular exchange between reproduc...

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Bibliographic Details
Published in:The Plant cell 2017-05, Vol.29 (5), p.984-1006
Main Authors: Leydon, Alexander R., Weinreb, Caleb, Venable, Elena, Reinders, Anke, Ward, John M., Johnson, Mark A.
Format: Article
Language:English
Subjects:
Animal reproduction
Arabidopsis
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Breeding success
Females
Fertilization
Flowering
Flowering plants
Gametes
Gene expression
Gene Expression Regulation, Plant - genetics
Gene polymorphism
Gene sequencing
Hybridization
Interspecific
Interspecific hybridization
Large-Scale Biology
LARGE-SCALE BIOLOGY ARTICLE
Molecular interactions
Parents
Plastics
Pollen
Polymorphism
Polymorphism, Single Nucleotide - genetics
Proteins
Reproduction
Reproduction (biology)
Ribonucleic acid
RNA
RNA, Plant - genetics
Sequence Analysis, RNA - methods
Sexual reproduction
Single-nucleotide polymorphism
Transcription factors
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Summary:The molecular interactions between reproductive cells are critical for determining whether sexual reproduction between individuals results in fertilization and can result in barriers to interspecific hybridization. However, it is a challenge to define the complete molecular exchange between reproductive partners because parents contribute to a complex mixture of cells during reproduction. We unambiguously defined male- and female-specific patterns of gene expression during Arabidopsis thaliana reproduction using single nucleotide polymorphism-informed RNA-sequencing analysis. Importantly, we defined the repertoire of pollen tube-secreted proteins controlled by a group of MYB transcription factors that are required for sperm release from the pollen tube to the female gametes, a critical barrier to interspecific hybridization. Our work defines the pollen tube gene products that respond to the pistil and are required for reproductive success; moreover, we find that these genes are highly evolutionarily plastic both at the level of coding sequence and expression across A. thaliana accessions.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.16.00816