Photoaffinity Labeling of the Human A2A Adenosine Receptor and Cross-link Position Analysis by Mass Spectrometry

Photoaffinity labeling (PAL) is widely used for the identification of ligand-binding proteins and elucidation of ligand-binding sites. PAL has also been employed for the characterization of G protein-coupled receptors (GPCRs); however, a limited number of reports has successfully identified their cr...

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Bibliographic Details
Published in:ACS medicinal chemistry letters 2017-06, Vol.8 (6), p.660-665
Main Authors: Muranaka, Hideyuki, Momose, Takaki, Handa, Chiaki, Ozawa, Tomonaga
Format: Article
Language:English
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Letter
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Summary:Photoaffinity labeling (PAL) is widely used for the identification of ligand-binding proteins and elucidation of ligand-binding sites. PAL has also been employed for the characterization of G protein-coupled receptors (GPCRs); however, a limited number of reports has successfully identified their cross-linked amino acids. This report is the first of its kind to determine the cross-link position of the human A2A adenosine receptor by PAL with the novel diazirine-based photoaffinity probe 9.
ISSN:1948-5875
1948-5875
DOI:10.1021/acsmedchemlett.7b00138