Serum Dried Samples to Detect Dengue Antibodies: A Field Study

Background. Dried blood and serum samples are useful resources for detecting antiviral antibodies. The conditions for elution of the sample need to be optimized for each disease. Dengue is a widespread disease in Mexico which requires continuous surveillance. In this study, we standardized and valid...

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Bibliographic Details
Published in:BioMed research international 2017-01, Vol.2017, p.7215259-6
Main Authors: Maldonado-Rodríguez, Angelica, Rojas-Montes, Othon, Vazquez-Rosales, Guillermo, Chavez-Negrete, Adolfo, Rojas-Uribe, Magdalena, Posadas-Mondragon, Araceli, Aguilar-Faisal, Leopoldo, Cevallos, Ana Maria, Xoconostle-Cazares, Beatriz, Lira, Rosalia
Format: Article
Language:English
Subjects:
Adult
Antibodies
Antibodies, Viral - blood
Cost reduction
Cross-Sectional Studies
Dengue
Dengue - blood
Dengue fever
Dengue Virus
Dried Blood Spot Testing - methods
Epidemiology
Female
Health aspects
Hospitals
Humans
Identification and classification
Immunoglobulins
Laboratories
Male
Mexico
Middle Aged
Patients
Physiological aspects
Public health
Studies
Viral antibodies
Viral infections
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Summary:Background. Dried blood and serum samples are useful resources for detecting antiviral antibodies. The conditions for elution of the sample need to be optimized for each disease. Dengue is a widespread disease in Mexico which requires continuous surveillance. In this study, we standardized and validated a protocol for the specific detection of dengue antibodies from dried serum spots (DSSs). Methods. Paired serum and DSS samples from 66 suspected cases of dengue were collected in a clinic in Veracruz, Mexico. Samples were sent to our laboratory, where the conditions for optimal elution of DSSs were established. The presence of anti-dengue antibodies was determined in the paired samples. Results. DSS elution conditions were standardized as follows: 1 h at 4°C in 200 µl of DNase-, RNase-, and protease-free PBS (1x). The optimal volume of DSS eluate to be used in the IgG assay was 40 µl. Sensitivity of 94%, specificity of 93.3%, and kappa concordance of 0.87 were obtained when comparing the antidengue reactivity between DSSs and serum samples. Conclusion. DSS samples are useful for detecting anti-dengue IgG antibodies in the field.
ISSN:2314-6133
2314-6141
DOI:10.1155/2017/7215259