Serum Dried Samples to Detect Dengue Antibodies: A Field Study
Background. Dried blood and serum samples are useful resources for detecting antiviral antibodies. The conditions for elution of the sample need to be optimized for each disease. Dengue is a widespread disease in Mexico which requires continuous surveillance. In this study, we standardized and valid...
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description | Background. Dried blood and serum samples are useful resources for detecting antiviral antibodies. The conditions for elution of the sample need to be optimized for each disease. Dengue is a widespread disease in Mexico which requires continuous surveillance. In this study, we standardized and validated a protocol for the specific detection of dengue antibodies from dried serum spots (DSSs). Methods. Paired serum and DSS samples from 66 suspected cases of dengue were collected in a clinic in Veracruz, Mexico. Samples were sent to our laboratory, where the conditions for optimal elution of DSSs were established. The presence of anti-dengue antibodies was determined in the paired samples. Results. DSS elution conditions were standardized as follows: 1 h at 4°C in 200 µl of DNase-, RNase-, and protease-free PBS (1x). The optimal volume of DSS eluate to be used in the IgG assay was 40 µl. Sensitivity of 94%, specificity of 93.3%, and kappa concordance of 0.87 were obtained when comparing the antidengue reactivity between DSSs and serum samples. Conclusion. DSS samples are useful for detecting anti-dengue IgG antibodies in the field. |
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Dried blood and serum samples are useful resources for detecting antiviral antibodies. The conditions for elution of the sample need to be optimized for each disease. Dengue is a widespread disease in Mexico which requires continuous surveillance. In this study, we standardized and validated a protocol for the specific detection of dengue antibodies from dried serum spots (DSSs). Methods. Paired serum and DSS samples from 66 suspected cases of dengue were collected in a clinic in Veracruz, Mexico. Samples were sent to our laboratory, where the conditions for optimal elution of DSSs were established. The presence of anti-dengue antibodies was determined in the paired samples. Results. DSS elution conditions were standardized as follows: 1 h at 4°C in 200 µl of DNase-, RNase-, and protease-free PBS (1x). The optimal volume of DSS eluate to be used in the IgG assay was 40 µl. Sensitivity of 94%, specificity of 93.3%, and kappa concordance of 0.87 were obtained when comparing the antidengue reactivity between DSSs and serum samples. Conclusion. DSS samples are useful for detecting anti-dengue IgG antibodies in the field.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2017/7215259</identifier><identifier>PMID: 28630868</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Adult ; Antibodies ; Antibodies, Viral - blood ; Cost reduction ; Cross-Sectional Studies ; Dengue ; Dengue - blood ; Dengue fever ; Dengue Virus ; Dried Blood Spot Testing - methods ; Epidemiology ; Female ; Health aspects ; Hospitals ; Humans ; Identification and classification ; Immunoglobulins ; Laboratories ; Male ; Mexico ; Middle Aged ; Patients ; Physiological aspects ; Public health ; Studies ; Viral antibodies ; Viral infections</subject><ispartof>BioMed research international, 2017-01, Vol.2017, p.7215259-6</ispartof><rights>Copyright © 2017 Angelica Maldonado-Rodríguez et al.</rights><rights>COPYRIGHT 2017 John Wiley & Sons, Inc.</rights><rights>Copyright © 2017 Angelica Maldonado-Rodríguez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2017 Angelica Maldonado-Rodríguez et al. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-d0357bcafa6cad51e458498425e4f0a4c6714b25f6db80e7d515f555f6cbd7a3</citedby><cites>FETCH-LOGICAL-c476t-d0357bcafa6cad51e458498425e4f0a4c6714b25f6db80e7d515f555f6cbd7a3</cites><orcidid>0000-0002-0753-7420 ; 0000-0002-7184-322X ; 0000-0002-6812-9471</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1908327518/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1908327518?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,25731,27901,27902,36989,36990,44566,74869</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28630868$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Domingo, Cristina</contributor><creatorcontrib>Maldonado-Rodríguez, Angelica</creatorcontrib><creatorcontrib>Rojas-Montes, Othon</creatorcontrib><creatorcontrib>Vazquez-Rosales, Guillermo</creatorcontrib><creatorcontrib>Chavez-Negrete, Adolfo</creatorcontrib><creatorcontrib>Rojas-Uribe, Magdalena</creatorcontrib><creatorcontrib>Posadas-Mondragon, Araceli</creatorcontrib><creatorcontrib>Aguilar-Faisal, Leopoldo</creatorcontrib><creatorcontrib>Cevallos, Ana Maria</creatorcontrib><creatorcontrib>Xoconostle-Cazares, Beatriz</creatorcontrib><creatorcontrib>Lira, Rosalia</creatorcontrib><title>Serum Dried Samples to Detect Dengue Antibodies: A Field Study</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Background. Dried blood and serum samples are useful resources for detecting antiviral antibodies. The conditions for elution of the sample need to be optimized for each disease. Dengue is a widespread disease in Mexico which requires continuous surveillance. In this study, we standardized and validated a protocol for the specific detection of dengue antibodies from dried serum spots (DSSs). Methods. Paired serum and DSS samples from 66 suspected cases of dengue were collected in a clinic in Veracruz, Mexico. Samples were sent to our laboratory, where the conditions for optimal elution of DSSs were established. The presence of anti-dengue antibodies was determined in the paired samples. Results. DSS elution conditions were standardized as follows: 1 h at 4°C in 200 µl of DNase-, RNase-, and protease-free PBS (1x). The optimal volume of DSS eluate to be used in the IgG assay was 40 µl. Sensitivity of 94%, specificity of 93.3%, and kappa concordance of 0.87 were obtained when comparing the antidengue reactivity between DSSs and serum samples. Conclusion. DSS samples are useful for detecting anti-dengue IgG antibodies in the field.</description><subject>Adult</subject><subject>Antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>Cost reduction</subject><subject>Cross-Sectional Studies</subject><subject>Dengue</subject><subject>Dengue - blood</subject><subject>Dengue fever</subject><subject>Dengue Virus</subject><subject>Dried Blood Spot Testing - methods</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Health aspects</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Identification and classification</subject><subject>Immunoglobulins</subject><subject>Laboratories</subject><subject>Male</subject><subject>Mexico</subject><subject>Middle Aged</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Public health</subject><subject>Studies</subject><subject>Viral antibodies</subject><subject>Viral infections</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNp90ctr3DAQB2BRWpqQ5tZzMfRSSLbRyHq5h8CSRxsI9JDchSyNNwq2tZXslvz30bLb7eMQXUZCHz9pGELeA_0MIMQZo6DOFAPBRPOKHLIa-EICh9f7fV0fkOOcH2lZGiRt5FtywLSsqZb6kJzfYZqH6jIF9NWdHdY95mqK1SVO6KZSxtWM1XKcQht9wPylWlbXAfuCp9k_vSNvOttnPN7VI3J_fXV_8W1x-_3rzcXyduG4ktPC01qo1tnOSme9AORC80ZzJpB31HInFfCWiU76VlNUhYhOiHJ2rVe2PiLn29j13A7oHY5Tsr1ZpzDY9GSiDebfmzE8mFX8aQSXiikoAZ92ASn-mDFPZgjZYd_bEeOcDTQAsmmYVoV-_I8-xjmNpbuiqK6ZEqD_qJXt0YSxi-Vdtwk1SyFUTZlkG3W6VS7FnBN2-y8DNZsBms0AzW6AhX_4u809_j2uAk624CGM3v4KL8c9Axbpn8U</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Maldonado-Rodríguez, Angelica</creator><creator>Rojas-Montes, Othon</creator><creator>Vazquez-Rosales, Guillermo</creator><creator>Chavez-Negrete, Adolfo</creator><creator>Rojas-Uribe, Magdalena</creator><creator>Posadas-Mondragon, Araceli</creator><creator>Aguilar-Faisal, Leopoldo</creator><creator>Cevallos, Ana Maria</creator><creator>Xoconostle-Cazares, Beatriz</creator><creator>Lira, Rosalia</creator><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0753-7420</orcidid><orcidid>https://orcid.org/0000-0002-7184-322X</orcidid><orcidid>https://orcid.org/0000-0002-6812-9471</orcidid></search><sort><creationdate>20170101</creationdate><title>Serum Dried Samples to Detect Dengue Antibodies: A Field Study</title><author>Maldonado-Rodríguez, Angelica ; Rojas-Montes, Othon ; Vazquez-Rosales, Guillermo ; Chavez-Negrete, Adolfo ; Rojas-Uribe, Magdalena ; Posadas-Mondragon, Araceli ; Aguilar-Faisal, Leopoldo ; Cevallos, Ana Maria ; Xoconostle-Cazares, Beatriz ; Lira, Rosalia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-d0357bcafa6cad51e458498425e4f0a4c6714b25f6db80e7d515f555f6cbd7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Antibodies</topic><topic>Antibodies, Viral - blood</topic><topic>Cost reduction</topic><topic>Cross-Sectional Studies</topic><topic>Dengue</topic><topic>Dengue - blood</topic><topic>Dengue fever</topic><topic>Dengue Virus</topic><topic>Dried Blood Spot Testing - methods</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Health aspects</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Identification and classification</topic><topic>Immunoglobulins</topic><topic>Laboratories</topic><topic>Male</topic><topic>Mexico</topic><topic>Middle Aged</topic><topic>Patients</topic><topic>Physiological aspects</topic><topic>Public health</topic><topic>Studies</topic><topic>Viral antibodies</topic><topic>Viral infections</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maldonado-Rodríguez, Angelica</creatorcontrib><creatorcontrib>Rojas-Montes, Othon</creatorcontrib><creatorcontrib>Vazquez-Rosales, Guillermo</creatorcontrib><creatorcontrib>Chavez-Negrete, Adolfo</creatorcontrib><creatorcontrib>Rojas-Uribe, Magdalena</creatorcontrib><creatorcontrib>Posadas-Mondragon, Araceli</creatorcontrib><creatorcontrib>Aguilar-Faisal, Leopoldo</creatorcontrib><creatorcontrib>Cevallos, Ana Maria</creatorcontrib><creatorcontrib>Xoconostle-Cazares, Beatriz</creatorcontrib><creatorcontrib>Lira, Rosalia</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Database (1962 - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maldonado-Rodríguez, Angelica</au><au>Rojas-Montes, Othon</au><au>Vazquez-Rosales, Guillermo</au><au>Chavez-Negrete, Adolfo</au><au>Rojas-Uribe, Magdalena</au><au>Posadas-Mondragon, Araceli</au><au>Aguilar-Faisal, Leopoldo</au><au>Cevallos, Ana Maria</au><au>Xoconostle-Cazares, Beatriz</au><au>Lira, Rosalia</au><au>Domingo, Cristina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum Dried Samples to Detect Dengue Antibodies: A Field Study</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>2017</volume><spage>7215259</spage><epage>6</epage><pages>7215259-6</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Background. Dried blood and serum samples are useful resources for detecting antiviral antibodies. The conditions for elution of the sample need to be optimized for each disease. Dengue is a widespread disease in Mexico which requires continuous surveillance. In this study, we standardized and validated a protocol for the specific detection of dengue antibodies from dried serum spots (DSSs). Methods. Paired serum and DSS samples from 66 suspected cases of dengue were collected in a clinic in Veracruz, Mexico. Samples were sent to our laboratory, where the conditions for optimal elution of DSSs were established. The presence of anti-dengue antibodies was determined in the paired samples. Results. DSS elution conditions were standardized as follows: 1 h at 4°C in 200 µl of DNase-, RNase-, and protease-free PBS (1x). The optimal volume of DSS eluate to be used in the IgG assay was 40 µl. Sensitivity of 94%, specificity of 93.3%, and kappa concordance of 0.87 were obtained when comparing the antidengue reactivity between DSSs and serum samples. Conclusion. DSS samples are useful for detecting anti-dengue IgG antibodies in the field.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>28630868</pmid><doi>10.1155/2017/7215259</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-0753-7420</orcidid><orcidid>https://orcid.org/0000-0002-7184-322X</orcidid><orcidid>https://orcid.org/0000-0002-6812-9471</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Adult Antibodies Antibodies, Viral - blood Cost reduction Cross-Sectional Studies Dengue Dengue - blood Dengue fever Dengue Virus Dried Blood Spot Testing - methods Epidemiology Female Health aspects Hospitals Humans Identification and classification Immunoglobulins Laboratories Male Mexico Middle Aged Patients Physiological aspects Public health Studies Viral antibodies Viral infections |
title | Serum Dried Samples to Detect Dengue Antibodies: A Field Study |
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