RABL2 interacts with the intraflagellar transport-B complex and CEP19 and participates in ciliary assembly

Proteins localized to the basal body and the centrosome play crucial roles in ciliary assembly and function. Although RABL2 and CEP19 are conserved in ciliated organisms and have been implicated in ciliary/flagellar functions, their roles are poorly understood. Here we show that RABL2 interacts with...

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Bibliographic Details
Published in:Molecular biology of the cell 2017-06, Vol.28 (12), p.1652-1666
Main Authors: Nishijima, Yuya, Hagiya, Yohei, Kubo, Tomohiro, Takei, Ryota, Katoh, Yohei, Nakayama, Kazuhisa
Format: Article
Language:English
Subjects:
Animals
Basal Bodies - metabolism
Biological Transport
Cell Cycle Proteins - metabolism
Centrioles - metabolism
Chlamydomonas reinhardtii - genetics
Chlamydomonas reinhardtii - metabolism
Cilia - metabolism
Flagella - metabolism
HEK293 Cells
Humans
Infertility, Male - genetics
Infertility, Male - metabolism
Male
Mice
Phenotype
Protein Binding
rab GTP-Binding Proteins - genetics
rab GTP-Binding Proteins - metabolism
Sperm Motility
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Summary:Proteins localized to the basal body and the centrosome play crucial roles in ciliary assembly and function. Although RABL2 and CEP19 are conserved in ciliated organisms and have been implicated in ciliary/flagellar functions, their roles are poorly understood. Here we show that RABL2 interacts with CEP19 and is recruited to the mother centriole and basal body in a CEP19-dependent manner and that CEP19 is recruited to the centriole probably via its binding to the centrosomal protein FGFR1OP. Disruption of the gene in results in the nonflagellated phenotype, suggesting a crucial role of RABL2 in ciliary/flagellar assembly. We also show that RABL2 interacts, in its GTP-bound state, with the intraflagellar transport (IFT)-B complex via the IFT74-IFT81 heterodimer and that the interaction is disrupted by a mutation found in male infertile mice ( mice) with a sperm flagella motility defect. Intriguingly, RABL2 binds to CEP19 and the IFT74-IFT81 heterodimer in a mutually exclusive manner. Furthermore, exogenous expression of the GDP-locked or -type RABL2 mutant in human cells results in mild defects in ciliary assembly. These results indicate that RABL2 localized to the basal body plays crucial roles in ciliary/flagellar assembly via its interaction with the IFT-B complex.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.E17-01-0017