Dominant mutations causing alterations in acetyl-coenzyme A carboxylase confer tolerance to cyclohexanedione and aryloxyphenoxypropionate herbicides in maize

• Published in: Proceedings of the National Academy of Sciences - PNAS 1990-09, Vol.87 (18), p.7175-7179
• Main Authors: Parker, W.B. (University of Minnesota, Saint Paul, MN), Marshall, L.C, Burton, J.D, Somers, D.A, Wyse, D.L, Gronwald, J.W, Gengenbach, B.G
• Format: Article
• Language: English
• Subjects: Acetyl-CoA Carboxylase - antagonists & inhibitors; Acetyl-CoA Carboxylase - genetics; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Agronomy. Soil science and plant productions; ARYLOXYPHENOXYPROPIONIC HERBICIDES; Biological and medical sciences; Callus; CARBOXY-LYASES; Cells, Cultured; Chemical control; CHEMICAL RESISTANCE; Corn; Crosses, Genetic; CULTIVO DE TEJIDOS; CULTURE DE TISSU; Cyclohexanes - pharmacology; Cyclohexanones - pharmacology; ENZYMIC ACTIVITY; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; Genes, Dominant; GENETIC INHERITANCE; Genetic mutation; GENOTIPOS; GENOTYPE; GENOTYPES; HERBICIDAS; HERBICIDE; Herbicide resistance; HERBICIDES; Herbicides - pharmacology; HEREDITE; HERENCIA GENETICA; Impact tolerances; Inbreeding; LIASAS; Ligases - genetics; LYASE; LYASES; MUTACION; MUTATION; Parasitic plants. Weeds; Phytopathology. Animal pests. Plant and forest protection; Plants; Pyridines - pharmacology; RESISTANCE AUX PRODUITS CHIMIQUES; RESISTENCIA QUIMICA; Seedlings; SELECCION; SELECTION; SETHOXYDIM; TISSUE CULTURE; Tissue culture techniques; Weeds; ZEA MAYS; Zea mays - drug effects; Zea mays - enzymology; Zea mays - genetics
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.87.18.7175

A partially dominant mutation exhibiting increased tolerance to cyclohexanedione and aryloxyphenoxypropionate herbicides was isolated by exposing susceptible maize (Zea mays) tissue cultures to increasingly inhibitory concentrations of sethoxydim (a cyclohexanedione). The selected tissue culture (S2) was >40-fold more tolerant to sethoxydim and 20-fold more tolerant to haloxyfop (an aryloxyphenoxypropionate) than the nonselected wild-type tissue culture. Regenerated S2 plants were heterozygous for the mutant allele and exhibited a high-level, but not complete, tolerance to both herbicides. Homozygous mutant families derived by self-pollinating the regenerated S2 plants exhibited no injury after treatment with 0.8 kg of sethoxydim per ha, which was >16-fold the rate lethal to wild-type plants. Acetyl-coenzyme A carboxylase (ACCase; EC 6.4.1.2) is the target enzyme of cyclohexanedione and aryloxyphenoxypropionate herbicides. ACCase activities of the nonselected wild-type and homozygous mutant seedlings were similar in the absence of herbicide. ACCase activity from homozygous tolerant plants required >100-fold more sethoxydim and 16-fold more haloxyfop for 50% inhibition than ACCase from wild-type plants. These results indicate that tolerance to sethoxydim and haloxyfop is controlled by a partially dominant nuclear mutation encoding a herbicide-insensitive alteration in maize ACCase.