Alanine-scanning mutagenesis of human STAT1 to estimate loss- or gain-of-function variants

Reference database based on the alanine-scanning assay is a useful tool for evaluating unknown variations in STAT1. Gain-of-function alanine mutants occurred at the interface of the antiparallel STAT1 dimer, suggesting that they destabilize this dimer.

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2016-12, Vol.140 (1), p.232-241
Main Authors: Kagawa, Reiko, Fujiki, Ryoji, Tsumura, Miyuki, Sakata, Sonoko, Nishimura, Shiho, Itan, Yuval, Kong, Xiao-Fei, Kato, Zenichiro, Ohnishi, Hidenori, Hirata, Osamu, Saito, Satoshi, Ikeda, Maiko, El Baghdadi, Jamila, Bousfiha, Aziz, Fujiwara, Kaori, Oleastro, Matias, Yancoski, Judith, Perez, Laura, Danielian, Silvia, Ailal, Fatima, Takada, Hidetoshi, Hara, Toshiro, Puel, Anne, Boisson-Dupuis, Stéphanie, Bustamante, Jacinta, Casanova, Jean-Laurent, Ohara, Osamu, Okada, Satoshi, Kobayashi, Masao
Format: Article
Language:English
Online Access:Get full text
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Summary:Reference database based on the alanine-scanning assay is a useful tool for evaluating unknown variations in STAT1. Gain-of-function alanine mutants occurred at the interface of the antiparallel STAT1 dimer, suggesting that they destabilize this dimer.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2016.09.035