Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons

Single-cell RNA sequencing (RNA-Seq) provides rich information about cell types and states. However, it is difficult to capture rare dynamic processes, such as adult neurogenesis, because isolation of rare neurons from adult tissue is challenging and markers for each phase are limited. Here, we deve...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2016-08, Vol.353 (6302), p.925-928
Main Authors: Habib, Naomi, Li, Yinqing, Heidenreich, Matthias, Swiech, Lukasz, Avraham-Davidi, Inbal, Trombetta, John J., Hession, Cynthia, Zhang, Feng, Regev, Aviv
Format: Article
Language:English
Subjects:
Adults
Algorithms
Animals
Cell Division - genetics
Cell growth
Cell Nucleus - metabolism
Cytoplasm
Deoxyuridine - analogs & derivatives
Deoxyuridine - analysis
Dynamics
Gene expression
Gene sequencing
Hippocampus - cytology
Hippocampus - metabolism
Individualized Instruction
Isotope Labeling
Marking
Mice
Neurogenesis
Neurogenesis - genetics
Neurons
Neurons - cytology
Neurons - metabolism
Nuclei
Ribonucleic acid
Ribonucleic acids
RNA
Sequence Analysis, RNA - methods
Single-Cell Analysis - methods
Spinal Cord - metabolism
Spinal Cord - pathology
Transcription, Genetic
Transcriptome
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Summary:Single-cell RNA sequencing (RNA-Seq) provides rich information about cell types and states. However, it is difficult to capture rare dynamic processes, such as adult neurogenesis, because isolation of rare neurons from adult tissue is challenging and markers for each phase are limited. Here, we develop Div-Seq, which combines scalable single-nucleus RNA-Seq (sNuc-Seq) with pulse labeling of proliferating cells by 5-ethynyl-2'-deoxyuridine (EdU) to profile individual dividing cells. sNuc-Seq and Div-Seq can sensitively identify closely related hippocampal cell types and track transcriptional dynamics of newborn neurons within the adult hippocampal neurogenic niche, respectively. We also apply Div-Seq to identify and profile rare newborn neurons in the adult spinal cord, a noncanonical neurogenic region. sNuc-Seq and Div-Seq open the way for unbiased analysis of diverse complex tissues.
ISSN:0036-8075
1095-9203
1095-9203
DOI:10.1126/science.aad7038