Inhibition of casein kinase 2 blocks G2/M transition in early embryo mitosis but not in oocyte meiosis in mouse

Casein kinase 2 (CK2) is a highly conserved, ubiquitously expressed serine/threonine protein kinase with hundreds of substrates. The role of CK2 in the G2/M transition of oocytes, zygotes, and 2-cell embryos was studied in mouse by enzyme activity inhibition using the specific inhibitor 4, 5, 6, 7-t...

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Bibliographic Details
Published in:Journal of Reproduction and Development 2017, Vol.63(3), pp.319-324
Main Authors: LIN, Fei, CAO, Shi-Bing, MA, Xue-Shan, SUN, Hai-Xiang
Format: Article
Language:English
Subjects:
4, 5, 6, 7-tetrabromobenzotriazole (TBB)
Casein
Casein kinase 2 (CK2)
Casein kinase II
DNA damage
Embryos
G2 phase
G2/M transition
Kinases
Meiosis
Mitosis
Oocytes
Original
Pronucleus
Protein kinase
Protein-serine/threonine kinase
Zygotes
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Summary:Casein kinase 2 (CK2) is a highly conserved, ubiquitously expressed serine/threonine protein kinase with hundreds of substrates. The role of CK2 in the G2/M transition of oocytes, zygotes, and 2-cell embryos was studied in mouse by enzyme activity inhibition using the specific inhibitor 4, 5, 6, 7-tetrabromobenzotriazole (TBB). Zygotes and 2-cell embryos were arrested at G2 phase by TBB treatment, and DNA damage was increased in the female pronucleus of arrested zygotes. Further developmental ability of arrested zygotes was reduced, but that of arrested 2-cell embryos was not affected after releasing from inhibition. By contrast, the G2/M transition in oocytes was not affected by TBB. These results indicate that CK2 activity is essential for mitotic G2/M transition in early embryos but not for meiotic G2/M transition in oocytes.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.2016-064