Expression of Salivary miR-203a-3p Was Related with Oral Health-Related Quality of Life in Healthy Volunteers

Oral health-related quality of life (OHRQoL) is a multidimensional construct that involves subjective evaluation of an individual's oral health. Although it is difficult to evaluate OHRQoL biologically, recently, it has been reported that circulating microRNAs (miRNAs) in several body fluids co...

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Bibliographic Details
Published in:International journal of molecular sciences 2017-06, Vol.18 (6), p.1263
Main Authors: Kobayashi, Terumasa, Tomofuji, Takaaki, Machida, Tatsuya, Yoneda, Toshiki, Ekuni, Daisuke, Azuma, Tetsuji, Maruyama, Takayuki, Hirose, Akiko, Morita, Manabu
Format: Article
Language:English
Subjects:
Adult
Arrays
Biomarkers
Body fluids
Cytokines
Evaluation
Female
Gene Expression
Gene Expression Regulation
Humans
Inflammation
Kinases
Male
MicroRNAs
MicroRNAs - genetics
miRNA
Oral diseases
Oral Health
Oral hygiene
Pain
Pilot Projects
Polymerase chain reaction
Population
Proteins
Quality of Life
Questionnaires
Real time
Saliva - metabolism
Signal Transduction
Students
Teeth
Up-Regulation
Young Adult
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Summary:Oral health-related quality of life (OHRQoL) is a multidimensional construct that involves subjective evaluation of an individual's oral health. Although it is difficult to evaluate OHRQoL biologically, recently, it has been reported that circulating microRNAs (miRNAs) in several body fluids could reflect various health conditions. The aim of this pilot study was to investigate whether salivary miRNAs expression differs according to OHRQoL in healthy volunteers. Forty-six volunteers (median age, 23.0 years) were recruited, and their OHRQoL was assessed using the Japanese version of the Oral Health Impact Profile (OHIP-J). Then, we compared salivary microRNA profiles of the high-OHRQoL group (≤25th percentile score of OHIP-J) and the low-OHRQoL group (≥75th percentile score of OHIP-J) using the polymerase chain reaction (PCR) array and the quantitative real-time PCR. There were no significant differences between the two groups in terms of oral health status. In the PCR array, miR-203a-3p and miR-30b-5p were significantly more expressed in the low-OHRQoL group ( < 0.05). Quantitative real-time PCR assay also showed that miR-203a-3p was more highly expressed in the low-OHRQoL group than in the high-OHRQoL group ( < 0.05). These observations suggest that expression of salivary miR-203a-3p was related with OHRQoL in healthy volunteers.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms18061263