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Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis
Guarana ( ) is a plant originated in Brazil that presents a beneficial effect on body weight control and metabolic alterations. The aim of this study was to evaluate the effects of guarana on genes and miRNAs related to adipogenesis in 3T3L1 cells. The anti-adipogenic effect of guarana was evaluated...
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| Published in: | Nutrients 2017-06, Vol.9 (6), p.635 |
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| container_title | Nutrients |
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| creator | Lima, Natália da Silva Numata, Erica de Paula Mesquita, Leonardo Mendes de Souza Dias, Pollyana Hammoud Vilegas, Wagner Gambero, Alessandra Ribeiro, Marcelo Lima |
| description | Guarana (
) is a plant originated in Brazil that presents a beneficial effect on body weight control and metabolic alterations. The aim of this study was to evaluate the effects of guarana on genes and miRNAs related to adipogenesis in 3T3L1 cells. The anti-adipogenic effect of guarana was evaluated by Oil Red-O staining. Gene and miRNA expression levels were determined by real time PCR. The Cebpα and β-catenin nuclear translocation were evaluated using immunocytochemistry. Our data indicated that the triglyceride-reducing effect of guarana was dose-dependent from 100 to 300 µg/mL (-12%, -20%, -24% and -40%, respectively,
< 0.0001). An up-regulation of the anti-adipogenic genes
,
,
,
and
and a down-regulation of pro-adipogenic genes
,
and
were also observed. Furthermore, guarana repressed mmu-miR-27b-3p, mmu-miR-34b-5p and mmu-miR-760-5p, that contributed for up-regulation of their molecular targets
,
and
. Additionally, cells treated with guarana presented an increase on β-catenin nuclear translocation (
< 0.0018). In summary, our data indicate that guarana has an anti-adipogenic potential due to its ability to modulate miRNAs and genes related to this process. Together our data demonstrate the important role of guarana as a putative therapeutic agent. |
| doi_str_mv | 10.3390/nu9060635 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5490614</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2271817126</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-ded1a898dde7ae3cabc97eb5daf7db3116d0ac48e14877c60908431ce003882d3</originalsourceid><addsrcrecordid>eNqFkU1LAzEQhoMottQe_AOy4KU9rOZjN8lexFJqFSp60HNIk2zdsk1qshH6793SWqoX5zLDzMPLzLwAXCJ4Q0gBb20sIIWU5CegiyHDKaUZOT2qO6AfwhJug0FGyTnoYE4JRkXRBffPTsdaNs5vkklZGtWExJXJNEovrUwGrzLWdWUrmai4bjvDxNlkpKu1WxhrQhUuwFkp62D6-9wD7w-Tt_FjOnuZPo1Hs1RlhDapNhpJXnCtDZOGKDlXBTPzXMuS6TlBiGooVcYNyjhjisIC8owgZSAknGNNeuBup7uO85XRytjGy1qsfbWSfiOcrMTvia0-xMJ9iTxr_4OyVmCwF_DuM5rQiFUVlKlraY2LQWDMEEcMYfovigrUgjmheYte_0GXLnrbfkJgBHlOc4ZISw13lPIuBG_Kw94Iiq2L4uBiy14dH3ogfzwj35IRlpc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2108565713</pqid></control><display><type>article</type><title>Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Lima, Natália da Silva ; Numata, Erica de Paula ; Mesquita, Leonardo Mendes de Souza ; Dias, Pollyana Hammoud ; Vilegas, Wagner ; Gambero, Alessandra ; Ribeiro, Marcelo Lima</creator><creatorcontrib>Lima, Natália da Silva ; Numata, Erica de Paula ; Mesquita, Leonardo Mendes de Souza ; Dias, Pollyana Hammoud ; Vilegas, Wagner ; Gambero, Alessandra ; Ribeiro, Marcelo Lima</creatorcontrib><description>Guarana (
) is a plant originated in Brazil that presents a beneficial effect on body weight control and metabolic alterations. The aim of this study was to evaluate the effects of guarana on genes and miRNAs related to adipogenesis in 3T3L1 cells. The anti-adipogenic effect of guarana was evaluated by Oil Red-O staining. Gene and miRNA expression levels were determined by real time PCR. The Cebpα and β-catenin nuclear translocation were evaluated using immunocytochemistry. Our data indicated that the triglyceride-reducing effect of guarana was dose-dependent from 100 to 300 µg/mL (-12%, -20%, -24% and -40%, respectively,
< 0.0001). An up-regulation of the anti-adipogenic genes
,
,
,
and
and a down-regulation of pro-adipogenic genes
,
and
were also observed. Furthermore, guarana repressed mmu-miR-27b-3p, mmu-miR-34b-5p and mmu-miR-760-5p, that contributed for up-regulation of their molecular targets
,
and
. Additionally, cells treated with guarana presented an increase on β-catenin nuclear translocation (
< 0.0018). In summary, our data indicate that guarana has an anti-adipogenic potential due to its ability to modulate miRNAs and genes related to this process. Together our data demonstrate the important role of guarana as a putative therapeutic agent.</description><identifier>ISSN: 2072-6643</identifier><identifier>EISSN: 2072-6643</identifier><identifier>DOI: 10.3390/nu9060635</identifier><identifier>PMID: 28632199</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>3T3-L1 Cells ; Adipogenesis ; Adipogenesis - drug effects ; Animals ; beta catenin ; Body weight ; Brazil ; Caffeine ; Chemical compounds ; dose response ; Dose-Response Relationship, Drug ; Flavonoids ; Gas flow ; GATA transcription factors ; GATA-3 protein ; Gene expression ; gene expression regulation ; Gene Expression Regulation - drug effects ; Gene regulation ; Genes ; Immunocytochemistry ; Laboratories ; Metabolism ; Mice ; microRNA ; miRNA ; Nuclear transport ; Obesity ; oils ; Paullinia - chemistry ; Paullinia cupana ; peroxisome proliferator-activated receptor gamma ; Pharmacology ; Plant Extracts - administration & dosage ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Preadipocyte factor 1 ; quantitative polymerase chain reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; staining ; therapeutics ; Translocation ; Weight control ; β-Catenin</subject><ispartof>Nutrients, 2017-06, Vol.9 (6), p.635</ispartof><rights>2017. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 by the authors. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-ded1a898dde7ae3cabc97eb5daf7db3116d0ac48e14877c60908431ce003882d3</citedby><cites>FETCH-LOGICAL-c436t-ded1a898dde7ae3cabc97eb5daf7db3116d0ac48e14877c60908431ce003882d3</cites><orcidid>0000-0001-9448-4090 ; 0000-0003-4529-7832 ; 0000-0003-3032-2556</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2108565713/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2108565713?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25731,27901,27902,36989,36990,44566,53766,53768,74869</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28632199$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lima, Natália da Silva</creatorcontrib><creatorcontrib>Numata, Erica de Paula</creatorcontrib><creatorcontrib>Mesquita, Leonardo Mendes de Souza</creatorcontrib><creatorcontrib>Dias, Pollyana Hammoud</creatorcontrib><creatorcontrib>Vilegas, Wagner</creatorcontrib><creatorcontrib>Gambero, Alessandra</creatorcontrib><creatorcontrib>Ribeiro, Marcelo Lima</creatorcontrib><title>Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis</title><title>Nutrients</title><addtitle>Nutrients</addtitle><description>Guarana (
) is a plant originated in Brazil that presents a beneficial effect on body weight control and metabolic alterations. The aim of this study was to evaluate the effects of guarana on genes and miRNAs related to adipogenesis in 3T3L1 cells. The anti-adipogenic effect of guarana was evaluated by Oil Red-O staining. Gene and miRNA expression levels were determined by real time PCR. The Cebpα and β-catenin nuclear translocation were evaluated using immunocytochemistry. Our data indicated that the triglyceride-reducing effect of guarana was dose-dependent from 100 to 300 µg/mL (-12%, -20%, -24% and -40%, respectively,
< 0.0001). An up-regulation of the anti-adipogenic genes
,
,
,
and
and a down-regulation of pro-adipogenic genes
,
and
were also observed. Furthermore, guarana repressed mmu-miR-27b-3p, mmu-miR-34b-5p and mmu-miR-760-5p, that contributed for up-regulation of their molecular targets
,
and
. Additionally, cells treated with guarana presented an increase on β-catenin nuclear translocation (
< 0.0018). In summary, our data indicate that guarana has an anti-adipogenic potential due to its ability to modulate miRNAs and genes related to this process. Together our data demonstrate the important role of guarana as a putative therapeutic agent.</description><subject>3T3-L1 Cells</subject><subject>Adipogenesis</subject><subject>Adipogenesis - drug effects</subject><subject>Animals</subject><subject>beta catenin</subject><subject>Body weight</subject><subject>Brazil</subject><subject>Caffeine</subject><subject>Chemical compounds</subject><subject>dose response</subject><subject>Dose-Response Relationship, Drug</subject><subject>Flavonoids</subject><subject>Gas flow</subject><subject>GATA transcription factors</subject><subject>GATA-3 protein</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Immunocytochemistry</subject><subject>Laboratories</subject><subject>Metabolism</subject><subject>Mice</subject><subject>microRNA</subject><subject>miRNA</subject><subject>Nuclear transport</subject><subject>Obesity</subject><subject>oils</subject><subject>Paullinia - chemistry</subject><subject>Paullinia cupana</subject><subject>peroxisome proliferator-activated receptor gamma</subject><subject>Pharmacology</subject><subject>Plant Extracts - administration & dosage</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Preadipocyte factor 1</subject><subject>quantitative polymerase chain reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>staining</subject><subject>therapeutics</subject><subject>Translocation</subject><subject>Weight control</subject><subject>β-Catenin</subject><issn>2072-6643</issn><issn>2072-6643</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqFkU1LAzEQhoMottQe_AOy4KU9rOZjN8lexFJqFSp60HNIk2zdsk1qshH6793SWqoX5zLDzMPLzLwAXCJ4Q0gBb20sIIWU5CegiyHDKaUZOT2qO6AfwhJug0FGyTnoYE4JRkXRBffPTsdaNs5vkklZGtWExJXJNEovrUwGrzLWdWUrmai4bjvDxNlkpKu1WxhrQhUuwFkp62D6-9wD7w-Tt_FjOnuZPo1Hs1RlhDapNhpJXnCtDZOGKDlXBTPzXMuS6TlBiGooVcYNyjhjisIC8owgZSAknGNNeuBup7uO85XRytjGy1qsfbWSfiOcrMTvia0-xMJ9iTxr_4OyVmCwF_DuM5rQiFUVlKlraY2LQWDMEEcMYfovigrUgjmheYte_0GXLnrbfkJgBHlOc4ZISw13lPIuBG_Kw94Iiq2L4uBiy14dH3ogfzwj35IRlpc</recordid><startdate>20170620</startdate><enddate>20170620</enddate><creator>Lima, Natália da Silva</creator><creator>Numata, Erica de Paula</creator><creator>Mesquita, Leonardo Mendes de Souza</creator><creator>Dias, Pollyana Hammoud</creator><creator>Vilegas, Wagner</creator><creator>Gambero, Alessandra</creator><creator>Ribeiro, Marcelo Lima</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9448-4090</orcidid><orcidid>https://orcid.org/0000-0003-4529-7832</orcidid><orcidid>https://orcid.org/0000-0003-3032-2556</orcidid></search><sort><creationdate>20170620</creationdate><title>Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis</title><author>Lima, Natália da Silva ; Numata, Erica de Paula ; Mesquita, Leonardo Mendes de Souza ; Dias, Pollyana Hammoud ; Vilegas, Wagner ; Gambero, Alessandra ; Ribeiro, Marcelo Lima</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-ded1a898dde7ae3cabc97eb5daf7db3116d0ac48e14877c60908431ce003882d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>3T3-L1 Cells</topic><topic>Adipogenesis</topic><topic>Adipogenesis - drug effects</topic><topic>Animals</topic><topic>beta catenin</topic><topic>Body weight</topic><topic>Brazil</topic><topic>Caffeine</topic><topic>Chemical compounds</topic><topic>dose response</topic><topic>Dose-Response Relationship, Drug</topic><topic>Flavonoids</topic><topic>Gas flow</topic><topic>GATA transcription factors</topic><topic>GATA-3 protein</topic><topic>Gene expression</topic><topic>gene expression regulation</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Gene regulation</topic><topic>Genes</topic><topic>Immunocytochemistry</topic><topic>Laboratories</topic><topic>Metabolism</topic><topic>Mice</topic><topic>microRNA</topic><topic>miRNA</topic><topic>Nuclear transport</topic><topic>Obesity</topic><topic>oils</topic><topic>Paullinia - chemistry</topic><topic>Paullinia cupana</topic><topic>peroxisome proliferator-activated receptor gamma</topic><topic>Pharmacology</topic><topic>Plant Extracts - administration & dosage</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Preadipocyte factor 1</topic><topic>quantitative polymerase chain reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>staining</topic><topic>therapeutics</topic><topic>Translocation</topic><topic>Weight control</topic><topic>β-Catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lima, Natália da Silva</creatorcontrib><creatorcontrib>Numata, Erica de Paula</creatorcontrib><creatorcontrib>Mesquita, Leonardo Mendes de Souza</creatorcontrib><creatorcontrib>Dias, Pollyana Hammoud</creatorcontrib><creatorcontrib>Vilegas, Wagner</creatorcontrib><creatorcontrib>Gambero, Alessandra</creatorcontrib><creatorcontrib>Ribeiro, Marcelo Lima</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Physical Education Index</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nutrients</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lima, Natália da Silva</au><au>Numata, Erica de Paula</au><au>Mesquita, Leonardo Mendes de Souza</au><au>Dias, Pollyana Hammoud</au><au>Vilegas, Wagner</au><au>Gambero, Alessandra</au><au>Ribeiro, Marcelo Lima</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis</atitle><jtitle>Nutrients</jtitle><addtitle>Nutrients</addtitle><date>2017-06-20</date><risdate>2017</risdate><volume>9</volume><issue>6</issue><spage>635</spage><pages>635-</pages><issn>2072-6643</issn><eissn>2072-6643</eissn><abstract>Guarana (
) is a plant originated in Brazil that presents a beneficial effect on body weight control and metabolic alterations. The aim of this study was to evaluate the effects of guarana on genes and miRNAs related to adipogenesis in 3T3L1 cells. The anti-adipogenic effect of guarana was evaluated by Oil Red-O staining. Gene and miRNA expression levels were determined by real time PCR. The Cebpα and β-catenin nuclear translocation were evaluated using immunocytochemistry. Our data indicated that the triglyceride-reducing effect of guarana was dose-dependent from 100 to 300 µg/mL (-12%, -20%, -24% and -40%, respectively,
< 0.0001). An up-regulation of the anti-adipogenic genes
,
,
,
and
and a down-regulation of pro-adipogenic genes
,
and
were also observed. Furthermore, guarana repressed mmu-miR-27b-3p, mmu-miR-34b-5p and mmu-miR-760-5p, that contributed for up-regulation of their molecular targets
,
and
. Additionally, cells treated with guarana presented an increase on β-catenin nuclear translocation (
< 0.0018). In summary, our data indicate that guarana has an anti-adipogenic potential due to its ability to modulate miRNAs and genes related to this process. Together our data demonstrate the important role of guarana as a putative therapeutic agent.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>28632199</pmid><doi>10.3390/nu9060635</doi><orcidid>https://orcid.org/0000-0001-9448-4090</orcidid><orcidid>https://orcid.org/0000-0003-4529-7832</orcidid><orcidid>https://orcid.org/0000-0003-3032-2556</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Nutrients, 2017-06, Vol.9 (6), p.635 |
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| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5490614 |
| source | Publicly Available Content Database; PubMed Central |
| subjects | 3T3-L1 Cells Adipogenesis Adipogenesis - drug effects Animals beta catenin Body weight Brazil Caffeine Chemical compounds dose response Dose-Response Relationship, Drug Flavonoids Gas flow GATA transcription factors GATA-3 protein Gene expression gene expression regulation Gene Expression Regulation - drug effects Gene regulation Genes Immunocytochemistry Laboratories Metabolism Mice microRNA miRNA Nuclear transport Obesity oils Paullinia - chemistry Paullinia cupana peroxisome proliferator-activated receptor gamma Pharmacology Plant Extracts - administration & dosage Plant Extracts - chemistry Plant Extracts - pharmacology Preadipocyte factor 1 quantitative polymerase chain reaction RNA, Messenger - genetics RNA, Messenger - metabolism staining therapeutics Translocation Weight control β-Catenin |
| title | Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis |
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