Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root Secretion

• Published in: Plant physiology (Bethesda) 2017-07, Vol.174 (3), p.1289-1306
• Main Authors: Pini, Francesco, East, Alison K., Appia-Ayme, Corinne, Tomek, Jakub, Karunakaran, Ramakrishnan, Mendoza-Suárez, Marcela, Edwards, Anne, Terpolilli, Jason J., Roworth, Joshua, Downie, J. Allan, Poole, Philip S.
• Format: Article
• Language: English
• Subjects: Biosensing Techniques; Breakthrough Technologies; Colony Count, Microbial; Gene Expression Regulation, Plant; Hesperidin - analysis; Image Processing, Computer-Assisted; Luminescence; Metabolome; Nitrogen Fixation; Pisum sativum - genetics; Pisum sativum - metabolism; Pisum sativum - microbiology; Plant Exudates - metabolism; Plant Root Nodulation; Plant Roots - genetics; Plant Roots - metabolism; Plant Roots - microbiology; Rhizobium leguminosarum - growth & development; Rhizobium leguminosarum - physiology; Rhizosphere; Root Nodules, Plant - microbiology; Symbiosis; Time Factors; Vicia - microbiology
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.16.01302

Plants engineer the rhizosphere to their advantage by secreting various nutrients and secondary metabolites. Coupling transcriptomic and metabolomic analyses of the pea (Pisum sativum) rhizosphere, a suite of bioreporters has been developed in Rhizobium leguminosarum bv viciae strain 3841, and these detect metabolites secreted by roots in space and time. Fourteen bacterial lux fusion bioreporters, specific for sugars, polyols, amino acids, organic acids, or flavonoids, have been validated in vitro and in vivo. Using different bacterial mutants (nodC and nifH), the process of colonization and symbiosis has been analyzed, revealing compounds important in the different steps of the rhizobium-legume association. Dicarboxylates and sucrose are the main carbon sources within the nodules; in ineffective (nifH) nodules, particularly low levels of sucrose were observed, suggesting that plant sanctions affect carbon supply to nodules. In contrast, high myo-inositol levels were observed prior to nodule formation and also in nifH senescent nodules. Amino acid biosensors showed different patterns: a g-aminobutyrate biosensor was active only inside nodules, whereas the phenylalanine bioreporter showed a high signal also in the rhizosphere. The bioreporters were further validated in vetch (Vicia hirsuta), producing similar results. In addition, vetch exhibited a local increase of nod gene-inducing flavonoids at sites where nodules developed subsequently. These bioreporters will be particularly helpful in understanding the dynamics of root exudation and the role of different molecules secreted into the rhizosphere.