Chicken egg shell as a potential substrate for production of alkaline protease by Bacillus altitudinis GVC11 and its applications

Chicken egg shell and membrane were used as substrate for production of alkaline protease by Bacillus altitudinis GVC11. Maltose as additional carbon source enhanced enzyme production up to 13%. Addition of organic nitrogen sources like peptone and yeast extract increased enzyme production by 9% and...

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Bibliographic Details
Published in:3 Biotech 2017-07, Vol.7 (3), p.185-6, Article 185
Main Authors: Nagamalli, Harikrishna, Sitaraman, Mahalakshmi, Kandalai, Kiran Kumar, Mudhole, Gopal Reddy
Format: Article
Language:English
Subjects:
Agriculture
Alanine
Alkaline protease
Amino acids
Antioxidants
Arginine
Bacillus altitudinis
Bioinformatics
Biomaterials
Biotechnology
Cancer Research
Carbon sources
Chemistry
Chemistry and Materials Science
Enzymes
Fermentation
Isoleucine
Leucine
Lysine
Maltose
Methionine
Nitrogen sources
Original
Original Article
Peptone
Protease
Stem Cells
Substrates
Valine
Yeast
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Summary:Chicken egg shell and membrane were used as substrate for production of alkaline protease by Bacillus altitudinis GVC11. Maltose as additional carbon source enhanced enzyme production up to 13%. Addition of organic nitrogen sources like peptone and yeast extract increased enzyme production by 9% and 5%, respectively and inorganic nitrogen sources did not have any positive effect. The resultant protein hydrolyzate after fermentation was found to have essential amino acids such as leucine, phenyl alanine, isoleucine, lysine, valine, methionine, arginine in considerable quantities and minute concentrations of cysteine. The protein hydrolyzate was also found to have good antioxidant activity.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-017-0801-y