Peri-adolescent asthma symptoms cause adult anxiety-related behavior and neurobiological processes in mice

• Published in: Behavioural brain research 2017-05, Vol.326, p.244-255
• Main Authors: Caulfield, Jasmine I., Caruso, Michael J., Michael, Kerry C., Bourne, Rebecca A., Chirichella, Nicole R., Klein, Laura C., Craig, Timothy, Bonneau, Robert H., August, Avery, Cavigelli, Sonia A.
• Format: Article
• Language: English
• Subjects: Adolescence; Age Factors; Animals; Anxiety; Anxiety - etiology; Asthma; Asthma - chemically induced; Asthma - complications; Asthma - immunology; Asthma - physiopathology; Behavior, Animal; Disease Models, Animal; Female; Gene Expression; Hippocampus - metabolism; House dust mite; Male; Methacholine; Methacholine Chloride - pharmacology; Mice; Mice, Inbred BALB C; Muscarinic Agonists - pharmacology; Pyroglyphidae - immunology; Sex differences; Sex Factors
• ISSN: 0166-4328; 1872-7549
• DOI: 10.1016/j.bbr.2017.02.046

•Experimental conditions induced asthma-like symptoms in adolescent mice.•Chronic adolescent asthma symptoms caused adult anxiety-related symptoms.•Induced labored breathing raised adult anxiety behavior and brain gene expression.•Induced airway inflammation led to decreased adult basal corticosterone production. Human and animal studies have shown that physical challenges and stressors during adolescence can have significant influences on behavioral and neurobiological development associated with internalizing disorders such as anxiety and depression. Given the prevalence of asthma during adolescence and increased rates of internalizing disorders in humans with asthma, we used a mouse model to test if and which symptoms of adolescent allergic asthma (airway inflammation or labored breathing) cause adult anxiety- and depression-related behavior and brain function. To mimic symptoms of allergic asthma in young BALB/cJ mice (postnatal days [P] 7–57; N=98), we induced lung inflammation with repeated intranasal administration of house dust mite extract (most common aeroallergen for humans) and bronchoconstriction with aerosolized methacholine (non-selective muscarinic receptor agonist). Three experimental groups, in addition to a control group, included: (1) “Airway inflammation only”, allergen exposure 3 times/week, (2) “Labored breathing only”, methacholine exposure once/week, and (3) “Airway inflammation+Labored breathing”, allergen and methacholine exposure. Compared to controls, mice that experienced methacholine-induced labored breathing during adolescence displayed a ∼20% decrease in time on open arms of the elevated plus maze in early adulthood (P60), a ∼30% decrease in brainstem serotonin transporter (SERT) mRNA expression and a ∼50% increase in hippocampal serotonin receptor 1a (5Htr1a) and corticotropin releasing hormone receptor 1 (Crhr1) expression in adulthood (P75). This is the first evidence that experimentally-induced clinical symptoms of adolescent asthma alter adult anxiety-related behavior and brain function several weeks after completion of asthma manipulations.