Identification of Protective B-Cell Epitopes within the Novel Malaria Vaccine Candidate Plasmodium falciparum Schizont Egress Antigen 1

Naturally acquired antibodies to schizont egress antigen 1 (PfSEA-1A) are associated with protection against severe malaria in children. Vaccination of mice with SEA-1A from (PbSEA-1A) decreases parasitemia and prolongs survival following ANKA challenge. To enhance the immunogenicity of PfSEA-1A, we...

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Bibliographic Details
Published in:Clinical and vaccine immunology 2017-07, Vol.24 (7)
Main Authors: Nixon, Christina E, Park, Sangshin, Pond-Tor, Sunthorn, Raj, Dipak, Lambert, Lynn E, Orr-Gonzalez, Sachy, Barnafo, Emma K, Rausch, Kelly M, Friedman, Jennifer F, Fried, Michal, Duffy, Patrick E, Kurtis, Jonathan D
Format: Article
Language:English
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Vaccines
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Summary:Naturally acquired antibodies to schizont egress antigen 1 (PfSEA-1A) are associated with protection against severe malaria in children. Vaccination of mice with SEA-1A from (PbSEA-1A) decreases parasitemia and prolongs survival following ANKA challenge. To enhance the immunogenicity of PfSEA-1A, we identified five linear B-cell epitopes using peptide microarrays probed with antisera from nonhuman primates vaccinated with recombinant PfSEA-1A (rPfSEA-1A). We evaluated the relationship between epitope-specific antibody levels and protection from parasitemia in a longitudinal treatment-reinfection cohort in western Kenya. Antibodies to three epitopes were associated with 16 to 17% decreased parasitemia over an 18-week high transmission season. We are currently designing immunogens to enhance antibody responses to these three epitopes.
ISSN:1556-6811
1556-679X
DOI:10.1128/CVI.00068-17