Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury

• Published in: Journal of Zhejiang University. B. Science 2017-07, Vol.18 (7), p.586-596
• Main Authors: Wang, Li-qin, He, Yu, Wan, Hao-fang, Zhou, Hui-fen, Yang, Jie-hong, Wan, Hai-tong
• Format: Article
• Language: English
• Subjects: 1-Phosphatidylinositol 3-kinase; Aconitine - analogs & derivatives; Aconitine - pharmacology; Aconitum; AKT protein; Animals; Annexin V; Anti-Inflammatory Agents - pharmacology; Apoptosis; Aspartate transaminase; BAX protein; Bcl-2 protein; Biomedical and Life Sciences; Biomedicine; Caspase; Caspase-9; Cell Survival; Compatibility; Creatine; Creatine kinase; Creatine Kinase, MB Form - metabolism; Deprivation; Electron microscopy; Enzyme-Linked Immunosorbent Assay; Fluorescein; Fluorescein isothiocyanate; Glucose; Glucose - metabolism; Glycyrrhetinic Acid - pharmacology; Heart Diseases - physiopathology; Injuries; Iodides; Kinases; L-Lactate dehydrogenase; L-Lactate Dehydrogenase - metabolism; Lactate dehydrogenase; Lactic acid; Microscopy, Electron, Transmission; Necrosis; Nuclei; Nuclei (cytology); Oxygen; Oxygen - metabolism; Phosphatidylinositol 3-Kinases - metabolism; Propidium iodide; Rats; Signal Transduction; Signaling; Staining; Transaminase; Transmission electron microscopy; Ultrastructure
• ISSN: 1673-1581; 1862-1783
• DOI: 10.1631/jzus.B1600270

This study investigated the protective effect of the compatibility of hypaconitine (HA) and glycyrrhetinic acid (GA) on H9c2 cells under oxygen and glucose deprivation (OGD)-induced injury, and the possible mechanisms. We found that HA+GA significantly improved pathology and morphology of the nucleus and ultrastructure of H9c2 cells under OGD as determined by Hoechst 33342 staining and transmission electron microscopy (TEM) tests. It also reduced the releases of lactate dehydrogenase (LDH), creatine kinase-myocardial band isoenzyme (CK-MB), and aspartate transaminase (AST) from the cultured supernatant of H9c2 cells, which were tested by enzyme-linked immune sorbent assay (ELISA) kits. In addition, it lessened the apoptotic rate as determined by a fluorescein isothiocyanate-annexin V/propidium iodide (FITC-AV/PI) double staining assay. It was also found that HA+GA might regulate the protein expression associated with the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. Overall, the study demonstrated that HA+GA protected H9c2 cells against OGD-induced injury, and the signaling mechanism might be related to the PI3K/Akt signaling pathway.