Embryonic Stem Cell Conditioned Medium Supports In Vitro Maturation of Mouse Oocytes

This study aimed to investigate the maturation and fertilization rates of immature mouse oocytes using Embryonic Stem Cell Conditioned Medium (ESCM). Germinal Vesicle (GV) stage oocytes were observed in 120 NMRI mice, aged 4-6 weeks. GV oocytes with or without cumulus cells were subjected to IVM in...

Full description

Saved in:
Bibliographic Details
Published in:Avicenna journal of medical biotechnology 2017-07, Vol.9 (3), p.114-119
Main Authors: Miraki, Saber, Mokarizadeh, Aram, Banafshi, Omid, Assadollahi, Vahideh, Abdi, Mahdad, Roshani, Daem, Fathi, Fardin
Format: Article
Language:English
Subjects:
Embryonic stem cells
Genetic aspects
Oocytes
Original
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study aimed to investigate the maturation and fertilization rates of immature mouse oocytes using Embryonic Stem Cell Conditioned Medium (ESCM). Germinal Vesicle (GV) stage oocytes were observed in 120 NMRI mice, aged 4-6 weeks. GV oocytes with or without cumulus cells were subjected to IVM in either ESCM, Embryonic Stem Cell Growth Medium (ESGM), or α-minimum essential medium (α-MEM). After recording the Metaphase II (MII) oocyte maturation rate, the oocytes were fertilized . The fertilization success rate was recorded after 24 . The embryos were maintained in potassium Simplex Optimization Medium (KSOM) for 96 and allowed to grow until the blastocyst stage. After recording developmental competence, they were transferred into the uteri of pseudopregnant mice and their birth rates were recorded. No significant difference existed between the maturation rates in α-MEM (68.18%) and ESCM (64.67%; p>0.05), whereas this rate was significantly higher for both α-MEM and ESCM compared to ESGM (32.22%; p
ISSN:2008-2835
2008-4625