Embryonic Stem Cell Conditioned Medium Supports In Vitro Maturation of Mouse Oocytes

This study aimed to investigate the maturation and fertilization rates of immature mouse oocytes using Embryonic Stem Cell Conditioned Medium (ESCM). Germinal Vesicle (GV) stage oocytes were observed in 120 NMRI mice, aged 4-6 weeks. GV oocytes with or without cumulus cells were subjected to IVM in...

Full description

Saved in:
Bibliographic Details
Published in:Avicenna journal of medical biotechnology 2017-07, Vol.9 (3), p.114-119
Main Authors: Miraki, Saber, Mokarizadeh, Aram, Banafshi, Omid, Assadollahi, Vahideh, Abdi, Mahdad, Roshani, Daem, Fathi, Fardin
Format: Article
Language:English
Subjects:
Embryonic stem cells
Genetic aspects
Oocytes
Original
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 119
container_issue 3
container_start_page 114
container_title Avicenna journal of medical biotechnology
container_volume 9
creator Miraki, Saber
Mokarizadeh, Aram
Banafshi, Omid
Assadollahi, Vahideh
Abdi, Mahdad
Roshani, Daem
Fathi, Fardin
description This study aimed to investigate the maturation and fertilization rates of immature mouse oocytes using Embryonic Stem Cell Conditioned Medium (ESCM). Germinal Vesicle (GV) stage oocytes were observed in 120 NMRI mice, aged 4-6 weeks. GV oocytes with or without cumulus cells were subjected to IVM in either ESCM, Embryonic Stem Cell Growth Medium (ESGM), or α-minimum essential medium (α-MEM). After recording the Metaphase II (MII) oocyte maturation rate, the oocytes were fertilized . The fertilization success rate was recorded after 24 . The embryos were maintained in potassium Simplex Optimization Medium (KSOM) for 96 and allowed to grow until the blastocyst stage. After recording developmental competence, they were transferred into the uteri of pseudopregnant mice and their birth rates were recorded. No significant difference existed between the maturation rates in α-MEM (68.18%) and ESCM (64.67%; p>0.05), whereas this rate was significantly higher for both α-MEM and ESCM compared to ESGM (32.22%; p
format article
fullrecord <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5501137</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A507012636</galeid><sourcerecordid>A507012636</sourcerecordid><originalsourceid>FETCH-LOGICAL-g248t-f8ed0b65672f93c011dd00ffa3b8907b653863fd466cef8dfa0fc51effd7b9413</originalsourceid><addsrcrecordid>eNptkV1LwzAUhosobsz9BQl4400lbZq0vRFGmTrY2MWmtyHNx4y0SU1TYf_ejE1xYHKRcM5z3pzz5iIapxAWcUZSfHm6pwXCo2ja9x_wsHKIMbqORmmRQ0IgHkfbeVu7vTWag42XLahk04DKGqG9tkYKsJJCDy3YDF1nne_BwoA37Z0FK-YHxw4UsAqs7NBLsLZ872V_E10p1vRyejon0evTfFu9xMv186KaLeNdmhU-VoUUsCaY5KkqEYdJIgSESjFUFyXMQwYVBCmREcKlKoRiUHGcSKVEXpdZgibR41G3G-pWCi6Nd6yhndMtc3tqmabnGaPf6c5-UYzDYygPAvcnAWc_B9l72uqeBwuYkWEgmpQpDD5mpAzo3RHdsUZSbZQNivyA0xkOxiYpQSRQD_9QYQvZah4MVTrEzwpu_47w2_vPD6FvWKCOoA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1920200469</pqid></control><display><type>article</type><title>Embryonic Stem Cell Conditioned Medium Supports In Vitro Maturation of Mouse Oocytes</title><source>Open Access: PubMed Central</source><creator>Miraki, Saber ; Mokarizadeh, Aram ; Banafshi, Omid ; Assadollahi, Vahideh ; Abdi, Mahdad ; Roshani, Daem ; Fathi, Fardin</creator><creatorcontrib>Miraki, Saber ; Mokarizadeh, Aram ; Banafshi, Omid ; Assadollahi, Vahideh ; Abdi, Mahdad ; Roshani, Daem ; Fathi, Fardin</creatorcontrib><description>This study aimed to investigate the maturation and fertilization rates of immature mouse oocytes using Embryonic Stem Cell Conditioned Medium (ESCM). Germinal Vesicle (GV) stage oocytes were observed in 120 NMRI mice, aged 4-6 weeks. GV oocytes with or without cumulus cells were subjected to IVM in either ESCM, Embryonic Stem Cell Growth Medium (ESGM), or α-minimum essential medium (α-MEM). After recording the Metaphase II (MII) oocyte maturation rate, the oocytes were fertilized . The fertilization success rate was recorded after 24 . The embryos were maintained in potassium Simplex Optimization Medium (KSOM) for 96 and allowed to grow until the blastocyst stage. After recording developmental competence, they were transferred into the uteri of pseudopregnant mice and their birth rates were recorded. No significant difference existed between the maturation rates in α-MEM (68.18%) and ESCM (64.67%; p&gt;0.05), whereas this rate was significantly higher for both α-MEM and ESCM compared to ESGM (32.22%; p&lt;0.05). A significant difference in IVF success rate existed for oocytes grown in α-MEM (69.44%), ESCM (61.53%), and ESGM (0%). A significantly higher developmental competence was observed at the blastocyst stage for oocytes grown in α-MEM (51.2%) compared to ESCM (35%; p&lt;0.05). 17 days after embryo transfer into the uteri of pseudopregnant mice, there was a nonsignficant (p&gt;0.05), similar birth rate between α-MEM and ESCM (47 . 40%). ESCM is an effective medium for preantral follicle growth, oocyte maturation, and subsequent embryo development.</description><identifier>ISSN: 2008-2835</identifier><identifier>EISSN: 2008-4625</identifier><identifier>PMID: 28706605</identifier><language>eng</language><publisher>Iran: Avicenna Research Institute</publisher><subject>Embryonic stem cells ; Genetic aspects ; Oocytes ; Original</subject><ispartof>Avicenna journal of medical biotechnology, 2017-07, Vol.9 (3), p.114-119</ispartof><rights>COPYRIGHT 2017 Avicenna Research Institute</rights><rights>Copyright© 2017 Avicenna Research Institute 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501137/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501137/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28706605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miraki, Saber</creatorcontrib><creatorcontrib>Mokarizadeh, Aram</creatorcontrib><creatorcontrib>Banafshi, Omid</creatorcontrib><creatorcontrib>Assadollahi, Vahideh</creatorcontrib><creatorcontrib>Abdi, Mahdad</creatorcontrib><creatorcontrib>Roshani, Daem</creatorcontrib><creatorcontrib>Fathi, Fardin</creatorcontrib><title>Embryonic Stem Cell Conditioned Medium Supports In Vitro Maturation of Mouse Oocytes</title><title>Avicenna journal of medical biotechnology</title><addtitle>Avicenna J Med Biotechnol</addtitle><description>This study aimed to investigate the maturation and fertilization rates of immature mouse oocytes using Embryonic Stem Cell Conditioned Medium (ESCM). Germinal Vesicle (GV) stage oocytes were observed in 120 NMRI mice, aged 4-6 weeks. GV oocytes with or without cumulus cells were subjected to IVM in either ESCM, Embryonic Stem Cell Growth Medium (ESGM), or α-minimum essential medium (α-MEM). After recording the Metaphase II (MII) oocyte maturation rate, the oocytes were fertilized . The fertilization success rate was recorded after 24 . The embryos were maintained in potassium Simplex Optimization Medium (KSOM) for 96 and allowed to grow until the blastocyst stage. After recording developmental competence, they were transferred into the uteri of pseudopregnant mice and their birth rates were recorded. No significant difference existed between the maturation rates in α-MEM (68.18%) and ESCM (64.67%; p&gt;0.05), whereas this rate was significantly higher for both α-MEM and ESCM compared to ESGM (32.22%; p&lt;0.05). A significant difference in IVF success rate existed for oocytes grown in α-MEM (69.44%), ESCM (61.53%), and ESGM (0%). A significantly higher developmental competence was observed at the blastocyst stage for oocytes grown in α-MEM (51.2%) compared to ESCM (35%; p&lt;0.05). 17 days after embryo transfer into the uteri of pseudopregnant mice, there was a nonsignficant (p&gt;0.05), similar birth rate between α-MEM and ESCM (47 . 40%). ESCM is an effective medium for preantral follicle growth, oocyte maturation, and subsequent embryo development.</description><subject>Embryonic stem cells</subject><subject>Genetic aspects</subject><subject>Oocytes</subject><subject>Original</subject><issn>2008-2835</issn><issn>2008-4625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNptkV1LwzAUhosobsz9BQl4400lbZq0vRFGmTrY2MWmtyHNx4y0SU1TYf_ejE1xYHKRcM5z3pzz5iIapxAWcUZSfHm6pwXCo2ja9x_wsHKIMbqORmmRQ0IgHkfbeVu7vTWag42XLahk04DKGqG9tkYKsJJCDy3YDF1nne_BwoA37Z0FK-YHxw4UsAqs7NBLsLZ872V_E10p1vRyejon0evTfFu9xMv186KaLeNdmhU-VoUUsCaY5KkqEYdJIgSESjFUFyXMQwYVBCmREcKlKoRiUHGcSKVEXpdZgibR41G3G-pWCi6Nd6yhndMtc3tqmabnGaPf6c5-UYzDYygPAvcnAWc_B9l72uqeBwuYkWEgmpQpDD5mpAzo3RHdsUZSbZQNivyA0xkOxiYpQSRQD_9QYQvZah4MVTrEzwpu_47w2_vPD6FvWKCOoA</recordid><startdate>201707</startdate><enddate>201707</enddate><creator>Miraki, Saber</creator><creator>Mokarizadeh, Aram</creator><creator>Banafshi, Omid</creator><creator>Assadollahi, Vahideh</creator><creator>Abdi, Mahdad</creator><creator>Roshani, Daem</creator><creator>Fathi, Fardin</creator><general>Avicenna Research Institute</general><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201707</creationdate><title>Embryonic Stem Cell Conditioned Medium Supports In Vitro Maturation of Mouse Oocytes</title><author>Miraki, Saber ; Mokarizadeh, Aram ; Banafshi, Omid ; Assadollahi, Vahideh ; Abdi, Mahdad ; Roshani, Daem ; Fathi, Fardin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g248t-f8ed0b65672f93c011dd00ffa3b8907b653863fd466cef8dfa0fc51effd7b9413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Embryonic stem cells</topic><topic>Genetic aspects</topic><topic>Oocytes</topic><topic>Original</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miraki, Saber</creatorcontrib><creatorcontrib>Mokarizadeh, Aram</creatorcontrib><creatorcontrib>Banafshi, Omid</creatorcontrib><creatorcontrib>Assadollahi, Vahideh</creatorcontrib><creatorcontrib>Abdi, Mahdad</creatorcontrib><creatorcontrib>Roshani, Daem</creatorcontrib><creatorcontrib>Fathi, Fardin</creatorcontrib><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Avicenna journal of medical biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miraki, Saber</au><au>Mokarizadeh, Aram</au><au>Banafshi, Omid</au><au>Assadollahi, Vahideh</au><au>Abdi, Mahdad</au><au>Roshani, Daem</au><au>Fathi, Fardin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Embryonic Stem Cell Conditioned Medium Supports In Vitro Maturation of Mouse Oocytes</atitle><jtitle>Avicenna journal of medical biotechnology</jtitle><addtitle>Avicenna J Med Biotechnol</addtitle><date>2017-07</date><risdate>2017</risdate><volume>9</volume><issue>3</issue><spage>114</spage><epage>119</epage><pages>114-119</pages><issn>2008-2835</issn><eissn>2008-4625</eissn><abstract>This study aimed to investigate the maturation and fertilization rates of immature mouse oocytes using Embryonic Stem Cell Conditioned Medium (ESCM). Germinal Vesicle (GV) stage oocytes were observed in 120 NMRI mice, aged 4-6 weeks. GV oocytes with or without cumulus cells were subjected to IVM in either ESCM, Embryonic Stem Cell Growth Medium (ESGM), or α-minimum essential medium (α-MEM). After recording the Metaphase II (MII) oocyte maturation rate, the oocytes were fertilized . The fertilization success rate was recorded after 24 . The embryos were maintained in potassium Simplex Optimization Medium (KSOM) for 96 and allowed to grow until the blastocyst stage. After recording developmental competence, they were transferred into the uteri of pseudopregnant mice and their birth rates were recorded. No significant difference existed between the maturation rates in α-MEM (68.18%) and ESCM (64.67%; p&gt;0.05), whereas this rate was significantly higher for both α-MEM and ESCM compared to ESGM (32.22%; p&lt;0.05). A significant difference in IVF success rate existed for oocytes grown in α-MEM (69.44%), ESCM (61.53%), and ESGM (0%). A significantly higher developmental competence was observed at the blastocyst stage for oocytes grown in α-MEM (51.2%) compared to ESCM (35%; p&lt;0.05). 17 days after embryo transfer into the uteri of pseudopregnant mice, there was a nonsignficant (p&gt;0.05), similar birth rate between α-MEM and ESCM (47 . 40%). ESCM is an effective medium for preantral follicle growth, oocyte maturation, and subsequent embryo development.</abstract><cop>Iran</cop><pub>Avicenna Research Institute</pub><pmid>28706605</pmid><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2008-2835
ispartof Avicenna journal of medical biotechnology, 2017-07, Vol.9 (3), p.114-119
issn 2008-2835
2008-4625
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5501137
source Open Access: PubMed Central
subjects Embryonic stem cells
Genetic aspects
Oocytes
Original
title Embryonic Stem Cell Conditioned Medium Supports In Vitro Maturation of Mouse Oocytes
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T13%3A12%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Embryonic%20Stem%20Cell%20Conditioned%20Medium%20Supports%20In%20Vitro%20Maturation%20of%20Mouse%20Oocytes&rft.jtitle=Avicenna%20journal%20of%20medical%20biotechnology&rft.au=Miraki,%20Saber&rft.date=2017-07&rft.volume=9&rft.issue=3&rft.spage=114&rft.epage=119&rft.pages=114-119&rft.issn=2008-2835&rft.eissn=2008-4625&rft_id=info:doi/&rft_dat=%3Cgale_pubme%3EA507012636%3C/gale_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-g248t-f8ed0b65672f93c011dd00ffa3b8907b653863fd466cef8dfa0fc51effd7b9413%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1920200469&rft_id=info:pmid/28706605&rft_galeid=A507012636&rfr_iscdi=true