Molecular Epidemiology of Malaria in Cameroon and Côte d'Ivoire. XXXI. Kelch 13 Propeller Sequences in Plasmodium falciparum Isolates before and after Implementation of Artemisinin-Based Combination Therapy

Artemisinin-resistant malaria has not been reported from Africa, but resistance can possibly spread from Asia or arise independently in Africa. The emergence of artemisinin resistance in Africa can be monitored by molecular assay of Kelch 13 (K13) propeller sequences. A total of 251 archived DNA sam...

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Published in:The American journal of tropical medicine and hygiene 2017-01, Vol.97 (1), p.222-224
Main Authors: Djaman, Joseph Allico, Olefongo, Dagnogo, Ako, Aristide Berenger, Roman, Jocelyne, Ngane, Vincent Foumane, Basco, Leonardo K, Tahar, Rachida
Format: Article
Language:English
Subjects:
Antimalarials - therapeutic use
Artemisinins - therapeutic use
Cameroon - epidemiology
Cote d'Ivoire - epidemiology
Gene Expression Regulation - drug effects
Human health and pathology
Humans
Infectious diseases
Life Sciences
Malaria, Falciparum - drug therapy
Malaria, Falciparum - parasitology
Molecular Epidemiology
Plasmodium falciparum - genetics
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Time Factors
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Summary:Artemisinin-resistant malaria has not been reported from Africa, but resistance can possibly spread from Asia or arise independently in Africa. The emergence of artemisinin resistance in Africa can be monitored by molecular assay of Kelch 13 (K13) propeller sequences. A total of 251 archived DNA samples of isolates collected in 2002, 2003, and 2006 in Yaounde, Cameroon, and 47 samples collected in 2006 and 2013 in Abidjan, Côte d'Ivoire, were analyzed for K13-propeller sequence polymorphism. Only one isolate carried a mutant K13-propeller allele (E602D). None of the isolates carried the key mutant alleles (Y493H, R539T, I543T, and C580Y) associated with artemisinin resistance in Cambodia. The presence of the mutant allele was not correlated with in vitro response to dihydroartemisinin determined by the classical hypoxanthine incorporation assay. There was no evidence of K13 mutations associated with artemisinin resistance before and soon after the introduction of artemisinin-based combination therapies in Cameroon and Côte d'Ivoire.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.16-0889