Catalase deletion promotes prediabetic phenotype in mice

• Published in: Free radical biology & medicine 2017-02, Vol.103, p.48-56
• Main Authors: Heit, Claire, Marshall, Stephanie, Singh, Surrendra, Yu, Xiaoqing, Charkoftaki, Georgia, Zhao, Hongyu, Orlicky, David J., Fritz, Kristofer S., Thompson, David C., Vasiliou, Vasilis
• Format: Article
• Language: English
• Subjects: Animals; Catalase; Catalase - genetics; Catalase - metabolism; Diabetes; Glucose Intolerance; Liver - enzymology; Liver - pathology; Male; Metabolism; Mice, Inbred C57BL; Mice, Knockout; Obesity; Obesity - enzymology; Organ Size; Oxidative Stress; Phenotype; Prediabetic State - enzymology; Prediabetic State - genetics; Steatosis
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2016.12.011

Hydrogen peroxide is produced endogenously and can be toxic to living organisms by inducing oxidative stress and cell damage. However, it has also been identified as a signal transduction molecule. By metabolizing hydrogen peroxide, catalase protects cells and tissues against oxidative damage and may also influence signal transduction mechanisms. Studies suggest that acatalasemic individuals (i.e., those with very low catalase activity) have a higher risk for the development of diabetes. We now report catalase knockout (Cat-/-) mice, when fed a normal (6.5% lipid) chow, exhibit an obese phenotype that manifests as an increase in body weight that becomes more pronounced with age. The mice demonstrate altered hepatic and muscle lipid deposition, as well as increases in serum and hepatic triglycerides (TGs), and increased hepatic transcription and protein expression of PPARγ. Liver morphology revealed steatosis with inflammation. Cat-/- mice also exhibited pancreatic morphological changes that correlated with impaired glucose tolerance and increased fasting serum insulin levels, conditions consistent with pre-diabetic status. RNA-seq analyses revealed a differential expression of pathways and genes in Cat-/- mice, many of which are related to metabolic syndrome, diabetes, and obesity, such as Pparg and Cidec. In conclusion, the results of the present study show mice devoid of catalase develop an obese, pre-diabetic phenotype and provide compelling evidence for catalase (or its products) being integral in metabolic regulation. •Catalase activity is integral to the regulation of systemic nutrient metabolism.•Catalase-deficient mice develop obesity and metabolic dysfunction on normal chow diet.•RNA-seq analyses support a dysregulated, obese phenotype in catalase-deficient mice.•Histopathologically, catalase-deficient mice are metabolically dysregulated and obese.