Ectopic expression of c‐jun leads to differentiation of P19 embryonal carcinoma cells

The product of the c‐jun proto‐oncogene, a major component of the transcription factor AP1, has been implicated in both positive and negative transcriptional control as well as in transformation. The role of c‐jun in early development and differentiation processes, however, is still largely unknown....

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Bibliographic Details
Published in:The EMBO journal 1990-06, Vol.9 (6), p.1831-1837
Main Authors: Groot, R.P., Kruyt, F.A., Saag, P.T., Kruijer, W.
Format: Article
Language:English
Subjects:
Biological and medical sciences
carcinoma
Carrier Proteins - genetics
Cell Differentiation - genetics
Cell differentiation, maturation, development, hematopoiesis
Cell Line
Cell physiology
DNA - genetics
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - genetics
Embryonal Carcinoma Stem Cells
Fundamental and applied biological sciences. Psychology
Humans
Molecular and cellular biology
Neoplastic Stem Cells - metabolism
P19 EC cells
Promoter Regions, Genetic - drug effects
Proto-Oncogene Mas
Proto-Oncogene Proteins - biosynthesis
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-jun
Proto-Oncogenes
Receptors, Retinoic Acid
Transcription Factors - biosynthesis
Transcription Factors - genetics
Transfection
Tretinoin - pharmacology
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Summary:The product of the c‐jun proto‐oncogene, a major component of the transcription factor AP1, has been implicated in both positive and negative transcriptional control as well as in transformation. The role of c‐jun in early development and differentiation processes, however, is still largely unknown. In this paper we show that ectopic expression of exogenous c‐jun sequences leads to differentiation and loss of the transformed phenotype of P19 embryonal carcinoma (EC) cells. The mixed populations of endoderm‐ and mesoderm‐like cells that were obtained after the introduction of c‐jun morphologically and biochemically resembled P19 cells differentiated in monolayer by retinoic acid (RA). Furthermore, we provide evidence that direct effects of the c‐jun gene product on transcription of the retinoic acid receptor beta (RAR beta) gene may be responsible for the differentiation‐promoting potential of c‐jun.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1990.tb08308.x