An evaluation of purified Salmonella Typhi protein antigens for the serological diagnosis of acute typhoid fever

Summary Objectives The diagnosis of typhoid fever is a challenge. Aiming to develop a typhoid diagnostic we measured antibody responses against Salmonella Typhi ( S . Typhi) protein antigens and the Vi polysaccharide in a cohort of Bangladeshi febrile patients. Methods IgM against 12 purified antige...

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Bibliographic Details
Published in:The Journal of infection 2017-08, Vol.75 (2), p.104-114
Main Authors: Tran Vu Thieu, Nga, Trinh Van, Tan, Tran Tuan, Anh, Klemm, Elizabeth J, Nguyen Ngoc Minh, Chau, Voong Vinh, Phat, Pham Thanh, Duy, Ho Ngoc Dan, Thanh, Pham Duc, Trung, Langat, Pinky, Martin, Laura B, Galan, Jorge, Liang, Li, Felgner, Philip L, Davies, D. Huw, de Jong, Hanna K, Maude, Rapeephan R, Fukushima, Masako, Wijedoru, Lalith, Ghose, Aniruddha, Samad, Rasheda, Dondorp, Arjen M, Faiz, Abul, Darton, Thomas C, Pollard, Andrew J, Thwaites, Guy E, Dougan, Gordon, Parry, Christopher M, Baker, Stephen
Format: Article
Language:English
Subjects:
Antibodies, Bacterial - blood
Antigens, Bacterial - immunology
Bacteriological Techniques - methods
Bangladesh
Diagnostics
Enteric fever
Febrile disease
Humans
IgM
Immunoglobulin M - blood
Infectious Disease
Polysaccharides, Bacterial - immunology
Salmonella Typhi
Salmonella typhi - immunology
Typhoid fever
Typhoid Fever - diagnosis
Typhoid Fever - immunology
Vi polysaccharide
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Summary:Summary Objectives The diagnosis of typhoid fever is a challenge. Aiming to develop a typhoid diagnostic we measured antibody responses against Salmonella Typhi ( S . Typhi) protein antigens and the Vi polysaccharide in a cohort of Bangladeshi febrile patients. Methods IgM against 12 purified antigens and the Vi polysaccharide was measured by ELISA in plasma from patients with confirmed typhoid fever (n = 32), other confirmed infections (n = 17), and healthy controls (n = 40). ELISAs with the most specific antigens were performed on plasma from 243 patients with undiagnosed febrile disease. Results IgM against the S . Typhi protein antigens correlated with each other (rho > 0.8), but not against Vi (rho 0.85, respectively. Applying a dynamic cut-off to patients with undiagnosed febrile disease suggested that 34–58% had an IgM response indicative of typhoid. Conclusions We evaluated the diagnostic potential of several S . Typhi antigens; our assays give good sensitivity and specificity, but require further assessment in differing patient populations.
ISSN:0163-4453
1532-2742
DOI:10.1016/j.jinf.2017.05.007