cis‐acting control elements for Krüppel expression in the Drosophila embryo

Krüppel (Kr), a gap gene of Drosophila, shows complex spatial patterns of expression during the different stages of embryogenesis. In order to identify cis‐acting sequences required for normal Kr gene expression, we analysed the expression patterns of fusion gene constructs in transgenic embryos. In...

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Bibliographic Details
Published in:The EMBO journal 1990-08, Vol.9 (8), p.2587-2595
Main Authors: Hoch, M., Schröder, C., Seifert, E., Jäckle, H.
Format: Article
Language:English
Subjects:
Animals
Antibodies
beta-Galactosidase - analysis
beta-Galactosidase - genetics
Biological and medical sciences
Blastoderm - physiology
DNA-Binding Proteins
Drosophila - embryology
Drosophila - genetics
Drosophila Proteins
Embryo, Nonmammalian - physiology
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation
Immunoenzyme Techniques
Juvenile Hormones - analysis
Juvenile Hormones - genetics
Kruppel-Like Transcription Factors
Molecular and cellular biology
Molecular genetics
Mutation
Recombinant Fusion Proteins - analysis
Repressor Proteins
Restriction Mapping
Transcription Factors
Transcription, Genetic
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Summary:Krüppel (Kr), a gap gene of Drosophila, shows complex spatial patterns of expression during the different stages of embryogenesis. In order to identify cis‐acting sequences required for normal Kr gene expression, we analysed the expression patterns of fusion gene constructs in transgenic embryos. In these constructs, bacterial lacZ expression was placed under the control of Kr sequences in front of a basal promoter. We identified cis‐acting Kr control units which drive beta‐galactosidase expression in 10 known locations of Kr expression in early and late embryos. More than one cis‐regulatory element drives the expression in the anterior domain at the blastoderm stage, in the nervous system, the midline precursor cells and in the amino‐serosa. In addition, two cis‐acting elements direct the first zygotic expression of Kr in a striped subpattern within the central region of the blastoderm embryo. Both elements respond to alterations in the activities of maternal organizer genes known to be required for Kr expression in establishing the thoracic and anterior abdominal segments in the wild‐type embryo.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1990.tb07440.x