Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice

• Published in: Bioscience reports 2017-08, Vol.37 (4)
• Main Authors: Zeng, Kun, Feng, Qi-Gao, Lin, Bao-Tao, Ma, Da-Hui, Liu, Chun-Min
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Cataract - genetics; Cataract - metabolism; Cataract - pathology; Cell Proliferation; Diabetes Complications - genetics; Diabetes Complications - metabolism; Diabetes Complications - pathology; Diabetes Mellitus, Experimental - genetics; Diabetes Mellitus, Experimental - metabolism; Diabetes Mellitus, Experimental - pathology; Epithelial Cells - metabolism; Epithelial Cells - pathology; Eye Proteins - genetics; Eye Proteins - metabolism; Lens Capsule, Crystalline - metabolism; Lens Capsule, Crystalline - pathology; Male; Mice; MicroRNAs - genetics; MicroRNAs - metabolism; Sirtuin 1 - genetics; Sirtuin 1 - metabolism
• ISSN: 0144-8463; 1573-4935; 1573-4935
• DOI: 10.1042/BSR20170695

Our study aimed at exploring the effects of on the proliferation and apoptosis of lens epithelial cells in diabetic cataract mice by targetting NAD -dependent histone deacetylase sirtulin 1 (SIRT1). Healthy male mice were assigned into normal and diabetic cataract groups. Blood glucose, lens turbidity, and apoptosis were measured. Lens epithelial cells were classified into the normal, blank, negative control (NC), mimics, inhibitors, siRNA-SIRT1, and inhibitors + siRNA-SIRT1 groups. , Bcl-2, Bax, p53, and SIRT1 expressions of each group were detected. Cell proliferation, cycle and apoptosis were tested by MTT assay and flow cytometry. can specifically bind to SIRT1 according to the luciferase system. SIRT1 protein concentration was strongly positive in normal mice and weakly positive in diabetic cataract mice. Apoptosis index of diabetic cataract mice was higher than the normal mice. Compared with normal mice, the expressions of , Bax, and p53 increased in diabetic cataract mice, while the Bcl-2 and SIRT1 expressions decreased. In comparison with the blank and NC groups, the expressions of , Bax, and p53 increased, while Bcl-2 and SIRT1 expressions decreased, and the proliferation decreased and apoptosis rate increased in the mimics and siRNA-SIRT1 groups; the results were contradicting for the inhibitor group. could promote apoptosis and inhibit proliferation of lens epithelial cells in diabetic cataract mice by targetting SIRT1.