Bacteriophage T4 late promoters: mapping 5′ ends of T4 gene 23 mRNAs

More than thirty 5′ ends of RNA molecules which transverse the bacteriophage T4 late gene, gene 23, have been mapped by nuclease protection and reverse transcription, to a 1.7 kilobase pair (kbp) region upstream of gene 23. Most of these 5′ ends arise from RNA processing and degradation. Two sites a...

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Bibliographic Details
Published in:The EMBO journal 1982-01, Vol.1 (1), p.107-114
Main Authors: Kassavetis, G.A., Geiduschek, E.P.
Format: Article
Language:English
Subjects:
Chromosome Mapping
Methyltransferases - metabolism
Multienzyme Complexes - metabolism
Nucleotidyltransferases - metabolism
Operon
phage T4
Phosphoric Monoester Hydrolases
RNA, Messenger - analysis
RNA, Viral - analysis
RNA-Directed DNA Polymerase - metabolism
T-Phages - genetics
Transcription, Genetic
Viral Proteins
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Summary:More than thirty 5′ ends of RNA molecules which transverse the bacteriophage T4 late gene, gene 23, have been mapped by nuclease protection and reverse transcription, to a 1.7 kilobase pair (kbp) region upstream of gene 23. Most of these 5′ ends arise from RNA processing and degradation. Two sites at which RNA synthesis is initiated have been identified by guanylylation of diphosphate‐ or triphosphate‐terminated RNA with vaccinia virion “capping” enzyme and are situated approximately 0.1 and 0.9 kbp upstream of gene 23. At least one other promoter lies more than 1.7 kbp upstream of gene 23, that is, outside of the mapped region.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1982.tb01132.x