Transient expression of the chicken lysozyme gene after transfer into human cells

The chicken lysozyme gene was inserted into an SV40‐based plasmid vector, and the recombinants were transfected into the human cell lines HeLa and MCF‐7. Correct and efficient transient expression directed by the lysozyme promoter was found in both of these cell lines, as determined by S1 nuclease m...

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Bibliographic Details
Published in:The EMBO journal 1982-01, Vol.1 (10), p.1207-1212
Main Authors: Matthias, P.D., Renkawitz, R., Grez, M., Schütz, G.
Format: Article
Language:English
Subjects:
Breast Neoplasms
Cell Line
Cloning, Molecular
DNA Restriction Enzymes
Female
Genes
HeLa Cells - enzymology
Humans
Kinetics
Muramidase - genetics
Plasmids
RNA, Messenger - genetics
Simian virus 40 - genetics
Transcription, Genetic
Transfection
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Summary:The chicken lysozyme gene was inserted into an SV40‐based plasmid vector, and the recombinants were transfected into the human cell lines HeLa and MCF‐7. Correct and efficient transient expression directed by the lysozyme promoter was found in both of these cell lines, as determined by S1 nuclease mapping and Northern blot analysis of the RNAs made. SV40 sequences dramatically enhance the expression of the lysozyme gene. This enhancing effect is only acting in cis and is distance/orientation dependent, since clones containing the lysozyme gene in either orientation produce different amounts of correct lysozyme transcripts. The transfected lysozyme gene was not induced by steroid hormone treatment of the cells.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1982.tb00014.x