Bronchial microdialysis monitoring of inflammatory response in open abdominal aortic aneurysm repair; an observational study

• Published in: Physiological reports 2017-07, Vol.5 (14), p.e13348-n/a
• Main Authors: Tyvold, Stig S., Dahl, Torbjørn, Dragsund, Stein, Gunnes, Sigurd, Lyng, Oddveig, Damås, Jan K., Aadahl, Petter, Solligård, Erik
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Aneurysms; Aorta; Aortic Aneurysm, Abdominal - surgery; Aortic aneurysms; Aortic surgery; Bronchoalveolar Lavage Fluid - chemistry; Bronchus; Cardiovascular Conditions, Disorders and Treatments; Catheters; Cytokines; Cytokines - analysis; Female; Humans; Immunology; Inflammation; Ischemia; Lung; Male; Microdialysis; Microdialysis - methods; Middle Aged; Monitoring, Intraoperative - methods; Observational studies; Original Research; Physiology; Postoperative Complications - diagnosis; Postoperative Complications - prevention & control; Reperfusion; reperfusion injury; Reperfusion Injury - diagnosis; Reperfusion Injury - prevention & control; Surgery
• ISSN: 2051-817X
• DOI: 10.14814/phy2.13348

Aortic surgery results in ischemia–reperfusion injury that induces an inflammatory response and frequent complications. The magnitude of the inflammatory response in blood and bronchi may be associated with the risk of immediate complications. The purpose of the study was to evaluate bronchial microdialysis as a continuous monitoring of cytokines in bronchial epithelial lining fluid (ELF) and to determine whether bronchial ELF cytokine levels reflect the ischemia–reperfusion injury and risk for complications during open abdominal aortic aneurysm (AAA) repair. We measured cytokines in venous blood using microdialysis and in serum for comparison. Sixteen patients scheduled for elective open AAA repair were included in a prospective observational study. Microdialysis catheters were introduced into a bronchi and a cubital vein. Eighteen cytokines were measured using a Bio‐Plex Magnetic Human Cytokine Panel. Samples were collected before and during cross‐clamping of the aorta as well as from 0 to 60 min and from 60 to 120 min of reperfusion. The ELF levels of several cytokines changed significantly during reperfusion. In particular, IL‐6 increased more than 10‐fold and IL‐13 more than 5‐fold during ischemia and reperfusion. Also, the venous levels of several inflammatory and anti‐inflammatory cytokines increased and exhibited their highest concentration during reperfusion. Both bronchial and venous cytokine levels correlated with duration of the procedure, intensive care days, and preoperative kidney disease. Three patients suffered organ failure as a direct consequence of the procedure, and in these patients the bronchial ELF concentrations of 17 of 18 cytokines differed significantly from patients without such complications. Bronchial microdialysis is suited for continuous monitoring of inflammation during open AAA repair. The bronchial ELF cytokine levels may be useful in predicting immediate complications such as organ failure in patients undergoing vascular surgery. Bronchial microdialysis is a safe method for monitoring the inflammatory responses during open abdominal aortic aneurysm repair. Levels of inflammatory cytokines in epithelial lining fluid may be predictors for immediate complications such as organ failure in patients undergoing vascular surgery.