Effects of Zinc Finger Protein A20 on Lipopolysaccharide (LPS)-Induced Pulmonary Inflammation/Anti-Inflammatory Mediators in an Acute Lung Injury/Acute Respiratory Distress Syndrome Rat Model

• Published in: Medical science monitor 2017-07, Vol.23, p.3536-3545
• Main Authors: Wu, Ding-Qian, Wu, Hong-Bo, Zhang, Mao, Wang, Jian-An
• Format: Article
• Language: English
• Subjects: Acute Lung Injury - drug therapy; Acute Lung Injury - metabolism; Animal Study; Animals; Anti-Inflammatory Agents - therapeutic use; Bronchoalveolar Lavage Fluid; Disease Models, Animal; Inflammation Mediators; Interleukin-10 - metabolism; Lipopolysaccharides - pharmacology; Lung - metabolism; Male; Neoplasm Proteins - drug effects; Neoplasm Proteins - metabolism; Neutrophils - metabolism; NF-kappa B - drug effects; NF-kappa B - metabolism; Nucleocytoplasmic Transport Proteins - drug effects; Nucleocytoplasmic Transport Proteins - metabolism; Pneumonia - chemically induced; Pneumonia - drug therapy; Pneumonia - metabolism; Rats; Rats, Sprague-Dawley; Respiratory Distress Syndrome, Adult - drug therapy; Respiratory Distress Syndrome, Adult - metabolism; Tumor Necrosis Factor alpha-Induced Protein 3 - metabolism; Tumor Necrosis Factor alpha-Induced Protein 3 - pharmacology; Tumor Necrosis Factor-alpha - drug effects; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1643-3750; 1234-1010; 1643-3750
• DOI: 10.12659/MSM.901700

BACKGROUND The aim of this study was to investigate the effects of zinc finger protein A20 on lipopolysaccharide (LPS)-induced pulmonary inflammation/anti-inflammatory mediators in an acute lung injury/acute respiratory distress syndrome (ALI/ARDS) rat model. MATERIAL AND METHODS Forty-eight ALI/ARDS rats were selected and assigned into normal saline (NS) (injected with NS), LPS (injected with LPS), LPS-C1 (injected with pEGFP-C1, NS and LPS), and A20 groups (injected with pEGFP-C1-A20, NS, and LPS). The wet/dry (W/D) ratio of rat lung tissues and total protein concentration and the number of neutrophils in bronchoalveolar lavage fluid (BALF) were detected. Enzyme-linked immunosorbent assay (ELISA) and qRT-PCR were applied to detect the protein and mRNA expressions of A20, IL-10, and TNF-α, respectively. Western blotting was employed to detect the protein expressions of A20, nuclear factor-kappa B (NF-κB) p65 and NF-κB p-P65 in rat lung tissues. RESULTS Compared with the NS group, the W/D ratio of rat lung tissues and total protein concentration and the number of neutrophils in BALF in the other 3 groups increased significantly. The protein and mRNA expressions of A20, IL-10, and TNF-α were significantly higher in the LPS group than in the NS group. The protein and mRNA expressions of A20 and IL-10 were significantly up-regulated and the expression of TNF-α, NF-κB p65, and NF-κB p-P65 was significantly down-regulated in rats injected with A20 compared to those in the LPS group. CONCLUSIONS The study provided evidence that zinc finger protein A20 can alleviate pulmonary inflammation by inhibiting TNF-α, NF-κB p65, and NF-κB p-P65 expressions and promoting IL-10 expression.