Perforin is present only in normal activated Lyt2+ T lymphocytes and not in L3T4+ cells, but the serine protease granzyme A is made by both subsets

We show that among the subsets of peripheral T lymphocytes (Lyt2+ L3T4‐ and L3T4+ Lyt2‐ cells) activated in short‐term cultures by stimulation with H‐2 incompatible leukocytes 97% of the cytolytic activity and all detectable perforin activity resides in the Lyt2+ cells. But both populations contain...

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Bibliographic Details
Published in:The EMBO journal 1987-04, Vol.6 (4), p.933-938
Main Authors: Garcia‐Sanz, J.A., Plaetinck, G., Velotti, F., Masson, D., Tschopp, J., MacDonald, H.R., Nabholz, M.
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Antigens, Ly - analysis
Biological and medical sciences
Cell interactions
Cells, Cultured
Cytoplasmic Granules - enzymology
Cytoplasmic Granules - ultrastructure
Cytotoxicity, Immunologic
Endopeptidases - metabolism
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Granzymes
Hemolysis
Immunobiology
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Serine Endopeptidases
T-Lymphocytes - classification
T-Lymphocytes - enzymology
T-Lymphocytes - immunology
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Summary:We show that among the subsets of peripheral T lymphocytes (Lyt2+ L3T4‐ and L3T4+ Lyt2‐ cells) activated in short‐term cultures by stimulation with H‐2 incompatible leukocytes 97% of the cytolytic activity and all detectable perforin activity resides in the Lyt2+ cells. But both populations contain approximately equal amounts of a serine protease, granzyme A, the expression of which was previously thought to be restricted to cytolytic T lymphocytes.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1987.tb04841.x