Perforin is present only in normal activated Lyt2+ T lymphocytes and not in L3T4+ cells, but the serine protease granzyme A is made by both subsets

We show that among the subsets of peripheral T lymphocytes (Lyt2+ L3T4‐ and L3T4+ Lyt2‐ cells) activated in short‐term cultures by stimulation with H‐2 incompatible leukocytes 97% of the cytolytic activity and all detectable perforin activity resides in the Lyt2+ cells. But both populations contain...

Full description

Saved in:
Bibliographic Details
Published in:The EMBO journal 1987-04, Vol.6 (4), p.933-938
Main Authors: Garcia‐Sanz, J.A., Plaetinck, G., Velotti, F., Masson, D., Tschopp, J., MacDonald, H.R., Nabholz, M.
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Antigens, Ly - analysis
Biological and medical sciences
Cell interactions
Cells, Cultured
Cytoplasmic Granules - enzymology
Cytoplasmic Granules - ultrastructure
Cytotoxicity, Immunologic
Endopeptidases - metabolism
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Granzymes
Hemolysis
Immunobiology
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Serine Endopeptidases
T-Lymphocytes - classification
T-Lymphocytes - enzymology
T-Lymphocytes - immunology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c4221-2601befbc61f1402c6420d3c23a369fdc873f656d7b682c122d6d1e35419f2153
cites
container_end_page 938
container_issue 4
container_start_page 933
container_title The EMBO journal
container_volume 6
creator Garcia‐Sanz, J.A.
Plaetinck, G.
Velotti, F.
Masson, D.
Tschopp, J.
MacDonald, H.R.
Nabholz, M.
description We show that among the subsets of peripheral T lymphocytes (Lyt2+ L3T4‐ and L3T4+ Lyt2‐ cells) activated in short‐term cultures by stimulation with H‐2 incompatible leukocytes 97% of the cytolytic activity and all detectable perforin activity resides in the Lyt2+ cells. But both populations contain approximately equal amounts of a serine protease, granzyme A, the expression of which was previously thought to be restricted to cytolytic T lymphocytes.
doi_str_mv 10.1002/j.1460-2075.1987.tb04841.x
format article
fullrecord <record><control><sourceid>wiley_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_553485</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>EMBJ04841</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4221-2601befbc61f1402c6420d3c23a369fdc873f656d7b682c122d6d1e35419f2153</originalsourceid><addsrcrecordid>eNqVkctu1DAYhS0EKkPLIyBZiF1J8C1OgsRiqMpNU7WLYW059u9ORrmMbE9peA1eGIcZjWDJypbPOf_FH0KvKckpIezdNqdCkoyRsshpXZV5bIioBM0fn6DFSXqKFoRJmgla1c_RixC2hJCiKukZOuOsLmUpFujXHXg3-nbAbcA7DwGGiMehm3B6Gkbf6w5rE9sHHcHi1RTZJV7jbup3m9FMEQLWg03GOPtXfC0usYGuC29xs484bgAHSNUh1R4j6AD43uvh59QDXs4te20BNxNuxrjBYd8EiOECPXO6C_DyeJ6j75-u11dfstXt569Xy1VmBGM0Y5LQBlxjJHVUEGakYMRyw7jmsnbWVCV3spC2bGTFDGXMSkuBF4LWjtGCn6MPh7q7fdODNWl1rzu1822v_aRG3ap_laHdqPvxQRUFF9Wcf3_IGz-G4MGdopSoGZTaqpmGmmmoGZQ6glKPKfzq7-an6JFM0t8cdR2M7lz6NdOGk61iJK1bJtvyYPvRdjD9xwDq-ubjtz93_htrzrKS</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Perforin is present only in normal activated Lyt2+ T lymphocytes and not in L3T4+ cells, but the serine protease granzyme A is made by both subsets</title><source>Open Access: PubMed Central</source><creator>Garcia‐Sanz, J.A. ; Plaetinck, G. ; Velotti, F. ; Masson, D. ; Tschopp, J. ; MacDonald, H.R. ; Nabholz, M.</creator><creatorcontrib>Garcia‐Sanz, J.A. ; Plaetinck, G. ; Velotti, F. ; Masson, D. ; Tschopp, J. ; MacDonald, H.R. ; Nabholz, M.</creatorcontrib><description>We show that among the subsets of peripheral T lymphocytes (Lyt2+ L3T4‐ and L3T4+ Lyt2‐ cells) activated in short‐term cultures by stimulation with H‐2 incompatible leukocytes 97% of the cytolytic activity and all detectable perforin activity resides in the Lyt2+ cells. But both populations contain approximately equal amounts of a serine protease, granzyme A, the expression of which was previously thought to be restricted to cytolytic T lymphocytes.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1002/j.1460-2075.1987.tb04841.x</identifier><identifier>PMID: 3297674</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>London: Nature Publishing Group</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Animals ; Antigens, Ly - analysis ; Biological and medical sciences ; Cell interactions ; Cells, Cultured ; Cytoplasmic Granules - enzymology ; Cytoplasmic Granules - ultrastructure ; Cytotoxicity, Immunologic ; Endopeptidases - metabolism ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Granzymes ; Hemolysis ; Immunobiology ; Lymphocyte Activation ; Mice ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Serine Endopeptidases ; T-Lymphocytes - classification ; T-Lymphocytes - enzymology ; T-Lymphocytes - immunology</subject><ispartof>The EMBO journal, 1987-04, Vol.6 (4), p.933-938</ispartof><rights>1987 European Molecular Biology Organization</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4221-2601befbc61f1402c6420d3c23a369fdc873f656d7b682c122d6d1e35419f2153</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC553485/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC553485/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=8206427$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3297674$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garcia‐Sanz, J.A.</creatorcontrib><creatorcontrib>Plaetinck, G.</creatorcontrib><creatorcontrib>Velotti, F.</creatorcontrib><creatorcontrib>Masson, D.</creatorcontrib><creatorcontrib>Tschopp, J.</creatorcontrib><creatorcontrib>MacDonald, H.R.</creatorcontrib><creatorcontrib>Nabholz, M.</creatorcontrib><title>Perforin is present only in normal activated Lyt2+ T lymphocytes and not in L3T4+ cells, but the serine protease granzyme A is made by both subsets</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><description>We show that among the subsets of peripheral T lymphocytes (Lyt2+ L3T4‐ and L3T4+ Lyt2‐ cells) activated in short‐term cultures by stimulation with H‐2 incompatible leukocytes 97% of the cytolytic activity and all detectable perforin activity resides in the Lyt2+ cells. But both populations contain approximately equal amounts of a serine protease, granzyme A, the expression of which was previously thought to be restricted to cytolytic T lymphocytes.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Antigens, Ly - analysis</subject><subject>Biological and medical sciences</subject><subject>Cell interactions</subject><subject>Cells, Cultured</subject><subject>Cytoplasmic Granules - enzymology</subject><subject>Cytoplasmic Granules - ultrastructure</subject><subject>Cytotoxicity, Immunologic</subject><subject>Endopeptidases - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Granzymes</subject><subject>Hemolysis</subject><subject>Immunobiology</subject><subject>Lymphocyte Activation</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred DBA</subject><subject>Serine Endopeptidases</subject><subject>T-Lymphocytes - classification</subject><subject>T-Lymphocytes - enzymology</subject><subject>T-Lymphocytes - immunology</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNqVkctu1DAYhS0EKkPLIyBZiF1J8C1OgsRiqMpNU7WLYW059u9ORrmMbE9peA1eGIcZjWDJypbPOf_FH0KvKckpIezdNqdCkoyRsshpXZV5bIioBM0fn6DFSXqKFoRJmgla1c_RixC2hJCiKukZOuOsLmUpFujXHXg3-nbAbcA7DwGGiMehm3B6Gkbf6w5rE9sHHcHi1RTZJV7jbup3m9FMEQLWg03GOPtXfC0usYGuC29xs484bgAHSNUh1R4j6AD43uvh59QDXs4te20BNxNuxrjBYd8EiOECPXO6C_DyeJ6j75-u11dfstXt569Xy1VmBGM0Y5LQBlxjJHVUEGakYMRyw7jmsnbWVCV3spC2bGTFDGXMSkuBF4LWjtGCn6MPh7q7fdODNWl1rzu1822v_aRG3ap_laHdqPvxQRUFF9Wcf3_IGz-G4MGdopSoGZTaqpmGmmmoGZQ6glKPKfzq7-an6JFM0t8cdR2M7lz6NdOGk61iJK1bJtvyYPvRdjD9xwDq-ubjtz93_htrzrKS</recordid><startdate>198704</startdate><enddate>198704</enddate><creator>Garcia‐Sanz, J.A.</creator><creator>Plaetinck, G.</creator><creator>Velotti, F.</creator><creator>Masson, D.</creator><creator>Tschopp, J.</creator><creator>MacDonald, H.R.</creator><creator>Nabholz, M.</creator><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>198704</creationdate><title>Perforin is present only in normal activated Lyt2+ T lymphocytes and not in L3T4+ cells, but the serine protease granzyme A is made by both subsets</title><author>Garcia‐Sanz, J.A. ; Plaetinck, G. ; Velotti, F. ; Masson, D. ; Tschopp, J. ; MacDonald, H.R. ; Nabholz, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4221-2601befbc61f1402c6420d3c23a369fdc873f656d7b682c122d6d1e35419f2153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Antigens, Ly - analysis</topic><topic>Biological and medical sciences</topic><topic>Cell interactions</topic><topic>Cells, Cultured</topic><topic>Cytoplasmic Granules - enzymology</topic><topic>Cytoplasmic Granules - ultrastructure</topic><topic>Cytotoxicity, Immunologic</topic><topic>Endopeptidases - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Granzymes</topic><topic>Hemolysis</topic><topic>Immunobiology</topic><topic>Lymphocyte Activation</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred DBA</topic><topic>Serine Endopeptidases</topic><topic>T-Lymphocytes - classification</topic><topic>T-Lymphocytes - enzymology</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garcia‐Sanz, J.A.</creatorcontrib><creatorcontrib>Plaetinck, G.</creatorcontrib><creatorcontrib>Velotti, F.</creatorcontrib><creatorcontrib>Masson, D.</creatorcontrib><creatorcontrib>Tschopp, J.</creatorcontrib><creatorcontrib>MacDonald, H.R.</creatorcontrib><creatorcontrib>Nabholz, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garcia‐Sanz, J.A.</au><au>Plaetinck, G.</au><au>Velotti, F.</au><au>Masson, D.</au><au>Tschopp, J.</au><au>MacDonald, H.R.</au><au>Nabholz, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Perforin is present only in normal activated Lyt2+ T lymphocytes and not in L3T4+ cells, but the serine protease granzyme A is made by both subsets</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>1987-04</date><risdate>1987</risdate><volume>6</volume><issue>4</issue><spage>933</spage><epage>938</epage><pages>933-938</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>We show that among the subsets of peripheral T lymphocytes (Lyt2+ L3T4‐ and L3T4+ Lyt2‐ cells) activated in short‐term cultures by stimulation with H‐2 incompatible leukocytes 97% of the cytolytic activity and all detectable perforin activity resides in the Lyt2+ cells. But both populations contain approximately equal amounts of a serine protease, granzyme A, the expression of which was previously thought to be restricted to cytolytic T lymphocytes.</abstract><cop>London</cop><pub>Nature Publishing Group</pub><pmid>3297674</pmid><doi>10.1002/j.1460-2075.1987.tb04841.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0261-4189
ispartof The EMBO journal, 1987-04, Vol.6 (4), p.933-938
issn 0261-4189
1460-2075
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_553485
source Open Access: PubMed Central
subjects Analysis of the immune response. Humoral and cellular immunity
Animals
Antigens, Ly - analysis
Biological and medical sciences
Cell interactions
Cells, Cultured
Cytoplasmic Granules - enzymology
Cytoplasmic Granules - ultrastructure
Cytotoxicity, Immunologic
Endopeptidases - metabolism
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Granzymes
Hemolysis
Immunobiology
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Serine Endopeptidases
T-Lymphocytes - classification
T-Lymphocytes - enzymology
T-Lymphocytes - immunology
title Perforin is present only in normal activated Lyt2+ T lymphocytes and not in L3T4+ cells, but the serine protease granzyme A is made by both subsets
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T20%3A14%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wiley_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Perforin%20is%20present%20only%20in%20normal%20activated%20Lyt2+%20T%20lymphocytes%20and%20not%20in%20L3T4+%20cells,%20but%20the%20serine%20protease%20granzyme%20A%20is%20made%20by%20both%20subsets&rft.jtitle=The%20EMBO%20journal&rft.au=Garcia%E2%80%90Sanz,%20J.A.&rft.date=1987-04&rft.volume=6&rft.issue=4&rft.spage=933&rft.epage=938&rft.pages=933-938&rft.issn=0261-4189&rft.eissn=1460-2075&rft.coden=EMJODG&rft_id=info:doi/10.1002/j.1460-2075.1987.tb04841.x&rft_dat=%3Cwiley_pubme%3EEMBJ04841%3C/wiley_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4221-2601befbc61f1402c6420d3c23a369fdc873f656d7b682c122d6d1e35419f2153%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/3297674&rfr_iscdi=true