Three‐dimensional structure of the large ribosomal subunit from Escherichia coli

The three‐dimensional structure of the large (50S) ribosomal subunit from Escherichia coli has been determined from electron micrographs of negatively stained specimens. A new method of three‐dimensional reconstruction was used which combines many images of individual subunits recorded at a single h...

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Bibliographic Details
Published in:The EMBO journal 1987-04, Vol.6 (4), p.1107-1114
Main Authors: Radermacher, M., Wagenknecht, T., Verschoor, A., Frank, J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Escherichia coli
Escherichia coli - ultrastructure
Fundamental and applied biological sciences. Psychology
Microbiology
Microscopy, Electron
Models, Structural
Morphology, structure, chemical composition
Mycology
Protein Conformation
Ribosomal Proteins - analysis
Ribosomes - ultrastructure
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Summary:The three‐dimensional structure of the large (50S) ribosomal subunit from Escherichia coli has been determined from electron micrographs of negatively stained specimens. A new method of three‐dimensional reconstruction was used which combines many images of individual subunits recorded at a single high tilt angle. A prominent feature of the reconstruction is a large groove on the side of the subunit that interacts with the small ribosomal subunit. This feature is probably of functional significance as it includes the regions where the peptidyl transferase site and the binding locations of the elongation factors have been mapped previously by immunoelectron microscopy.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1987.tb04865.x