The bovine leukemia virus p34 is a transactivator protein

Recombinant Moloney murine retroviruses containing the BLV post‐envelope long open reading frame were constructed and transfected into the psi 2 packaging cell line. They were shown to encode and to express a 34‐kd protein able to transactivate the BLV long terminal repeat‐directed gene expression i...

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Bibliographic Details
Published in:The EMBO journal 1987-11, Vol.6 (11), p.3385-3389
Main Authors: Willems, L., Gegonne, A., Chen, G., Burny, A., Kettmann, R., Ghysdael, J.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Cell Line
DNA - analysis
Fundamental and applied biological sciences. Psychology
Gene expression
Genes
Genes, Viral
Genetic Vectors
Leukemia Virus, Bovine - genetics
Life sciences
Molecular and cellular biology
Molecular genetics
Médecine vétérinaire & santé animale
Retroviridae - genetics
Sciences du vivant
Transcription Factors - genetics
Transfection
Veterinary medicine & animal health
Viral Envelope Proteins - genetics
Viral Proteins - metabolism
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Summary:Recombinant Moloney murine retroviruses containing the BLV post‐envelope long open reading frame were constructed and transfected into the psi 2 packaging cell line. They were shown to encode and to express a 34‐kd protein able to transactivate the BLV long terminal repeat‐directed gene expression in the respective transfected cells. These data demonstrate that the BLV X‐LOR gene encodes a p34 transactivator product. Furthermore, the different cell lines produced infectious recombinant retroviruses capable of transferring X‐LOR genes into recipient cells. The availability of the BLV transactivator protein should allow us to understand the role of the transactivator protein in BLV‐induced leukemogenesis.
ISSN:0261-4189
1460-2075
1460-2075
DOI:10.1002/j.1460-2075.1987.tb02661.x