An efficient ribosomal frame‐shifting signal in the polymerase‐encoding region of the coronavirus IBV

The polymerase‐encoding region of the genomic RNA of the coronavirus infectious bronchitis virus (IBV) contains two very large, briefly overlapping open reading frames (ORF), F1 and F2, and it has been suggested on the basis of sequence analysis that expression of the downstream ORF, F2, might be me...

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Bibliographic Details
Published in:The EMBO journal 1987-12, Vol.6 (12), p.3779-3785
Main Authors: Brierley, I., Boursnell, M. E., Binns, M. M., Bilimoria, B., Blok, V. C., Brown, T. D., Inglis, S. C.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Coronaviridae - enzymology
Coronaviridae - genetics
DNA-Directed RNA Polymerases - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Viral
Genetic Vectors
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Conformation
Promoter Regions, Genetic
Protein Biosynthesis
Ribosomes - metabolism
RNA, Messenger - genetics
RNA, Viral - genetics
Transcription, Genetic
Translation. Translation factors. Protein processing
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Summary:The polymerase‐encoding region of the genomic RNA of the coronavirus infectious bronchitis virus (IBV) contains two very large, briefly overlapping open reading frames (ORF), F1 and F2, and it has been suggested on the basis of sequence analysis that expression of the downstream ORF, F2, might be mediated through ribosomal frame‐shifting. To examine this possibility a cDNA fragment containing the F1/F2 overlap region was cloned within a marker gene and placed under the control of the bacteriophage SP6 promoter in a recombinant plasmid. Messenger RNA transcribed from this plasmid, when translated in cell‐free systems, specified the synthesis of polypeptides whose size was entirely consistent with the products predicted by an efficient ribosomal frame‐shifting event within the overlap region. The nature of the products was confirmed by their reactivity with antisera raised against defined portions of the flanking marker gene. This is the first non‐retroviral example of ribosomal frame‐shifting in higher eukaryotes.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1987.tb02713.x