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Re-using blood products as an alternative supplement in the optimisation of clinical-grade adipose-derived mesenchymal stem cell culture
Adipose-derived mesenchymal stem cells (ADMSCs) are a promising strategy for orthopaedic applications, particularly in bone repair. expansion of ADMSCs is required to obtain sufficient cell numbers. Xenogenic supplements should be avoided in order to minimise the risk of infections and immunological...
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| Published in: | Bone & joint research 2017-07, Vol.6 (7), p.414-422 |
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| container_title | Bone & joint research |
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| creator | Phetfong, J Tawonsawatruk, T Seenprachawong, K Srisarin, A Isarankura-Na-Ayudhya, C Supokawej, A |
| description | Adipose-derived mesenchymal stem cells (ADMSCs) are a promising strategy for orthopaedic applications, particularly in bone repair.
expansion of ADMSCs is required to obtain sufficient cell numbers. Xenogenic supplements should be avoided in order to minimise the risk of infections and immunological reactions. Human platelet lysate and human plasma may be an excellent material source for ADMSC expansion. In the present study, use of blood products after their recommended transfusion date to prepare human platelet lysate (HPL) and human plasma (Hplasma) was evaluated for
culture expansion and osteogenesis of ADMSCs.
Human ADMSCs were cultured in medium supplemented with HPL, Hplasma and a combination of HPL and Hplasma (HPL+Hplasma). Characteristics of these ADMSCs, including osteogenesis, were evaluated in comparison with those cultured in fetal bovine serum (FBS).
HPL and HPL+Hplasma had a significantly greater growth-promoting effect than FBS, while Hplasma exhibited a similar growth-promoting effect to that of FBS. ADMSCs cultured in HPL and/or Hplasma generated more colony-forming unit fibroblasts (CFU-F) than those cultured in FBS. After long-term culture, ADMSCs cultured in HPL and/or Hplasma showed reduced cellular senescence, retained typical cell phenotypes, and retained differentiation capacities into osteogenic and adipogenic lineages.
HPL and Hplasma prepared from blood products after their recommended transfusion date can be used as an alternative and effective source for large-scale
expansion of ADMSCs.
J. Phetfong, T. Tawonsawatruk, K. Seenprachawong, A. Srisarin, C. Isarankura-Na-Ayudhya, A. Supokawej. Re-using blood products as an alternative supplement in the optimisation of clinical-grade adipose-derived mesenchymal stem cell culture. Bone Joint Res 2017;6:414-422. DOI: 10.1302/2046-3758.67.BJR-2016-0342.R1. |
| doi_str_mv | 10.1302/2046-3758.67.BJR-2016-0342.R1 |
| format | article |
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expansion of ADMSCs is required to obtain sufficient cell numbers. Xenogenic supplements should be avoided in order to minimise the risk of infections and immunological reactions. Human platelet lysate and human plasma may be an excellent material source for ADMSC expansion. In the present study, use of blood products after their recommended transfusion date to prepare human platelet lysate (HPL) and human plasma (Hplasma) was evaluated for
culture expansion and osteogenesis of ADMSCs.
Human ADMSCs were cultured in medium supplemented with HPL, Hplasma and a combination of HPL and Hplasma (HPL+Hplasma). Characteristics of these ADMSCs, including osteogenesis, were evaluated in comparison with those cultured in fetal bovine serum (FBS).
HPL and HPL+Hplasma had a significantly greater growth-promoting effect than FBS, while Hplasma exhibited a similar growth-promoting effect to that of FBS. ADMSCs cultured in HPL and/or Hplasma generated more colony-forming unit fibroblasts (CFU-F) than those cultured in FBS. After long-term culture, ADMSCs cultured in HPL and/or Hplasma showed reduced cellular senescence, retained typical cell phenotypes, and retained differentiation capacities into osteogenic and adipogenic lineages.
HPL and Hplasma prepared from blood products after their recommended transfusion date can be used as an alternative and effective source for large-scale
expansion of ADMSCs.
J. Phetfong, T. Tawonsawatruk, K. Seenprachawong, A. Srisarin, C. Isarankura-Na-Ayudhya, A. Supokawej. Re-using blood products as an alternative supplement in the optimisation of clinical-grade adipose-derived mesenchymal stem cell culture. Bone Joint Res 2017;6:414-422. DOI: 10.1302/2046-3758.67.BJR-2016-0342.R1.</description><identifier>ISSN: 2046-3758</identifier><identifier>EISSN: 2046-3758</identifier><identifier>DOI: 10.1302/2046-3758.67.BJR-2016-0342.R1</identifier><identifier>PMID: 28720606</identifier><language>eng</language><publisher>England: British Editorial Society of Bone & Joint Surgery</publisher><subject>Adipose-Derived Mesenchymal Stem Cells ; Blood products ; Bone healing ; Cell culture ; Cell differentiation ; Dietary supplements ; Fetal calf serum ; Fibroblasts ; Human Plasma ; Mesenchymal Stem Cells ; Mesenchyme ; Osteogenesis ; Phenotypes ; Platelet Lysate ; Platelets ; Senescence ; Stem Cell Culture ; Stem cells</subject><ispartof>Bone & joint research, 2017-07, Vol.6 (7), p.414-422</ispartof><rights>2017 Supokawej et al.</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Supokawej et al. 2017 BJR Publishing Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-6af506a83280c29131976e5fe9277a61bc429ebdd53529dfb75e7e4aa5dc20693</citedby><cites>FETCH-LOGICAL-c453t-6af506a83280c29131976e5fe9277a61bc429ebdd53529dfb75e7e4aa5dc20693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539302/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539302/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28720606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Phetfong, J</creatorcontrib><creatorcontrib>Tawonsawatruk, T</creatorcontrib><creatorcontrib>Seenprachawong, K</creatorcontrib><creatorcontrib>Srisarin, A</creatorcontrib><creatorcontrib>Isarankura-Na-Ayudhya, C</creatorcontrib><creatorcontrib>Supokawej, A</creatorcontrib><title>Re-using blood products as an alternative supplement in the optimisation of clinical-grade adipose-derived mesenchymal stem cell culture</title><title>Bone & joint research</title><addtitle>Bone Joint Res</addtitle><description>Adipose-derived mesenchymal stem cells (ADMSCs) are a promising strategy for orthopaedic applications, particularly in bone repair.
expansion of ADMSCs is required to obtain sufficient cell numbers. Xenogenic supplements should be avoided in order to minimise the risk of infections and immunological reactions. Human platelet lysate and human plasma may be an excellent material source for ADMSC expansion. In the present study, use of blood products after their recommended transfusion date to prepare human platelet lysate (HPL) and human plasma (Hplasma) was evaluated for
culture expansion and osteogenesis of ADMSCs.
Human ADMSCs were cultured in medium supplemented with HPL, Hplasma and a combination of HPL and Hplasma (HPL+Hplasma). Characteristics of these ADMSCs, including osteogenesis, were evaluated in comparison with those cultured in fetal bovine serum (FBS).
HPL and HPL+Hplasma had a significantly greater growth-promoting effect than FBS, while Hplasma exhibited a similar growth-promoting effect to that of FBS. ADMSCs cultured in HPL and/or Hplasma generated more colony-forming unit fibroblasts (CFU-F) than those cultured in FBS. After long-term culture, ADMSCs cultured in HPL and/or Hplasma showed reduced cellular senescence, retained typical cell phenotypes, and retained differentiation capacities into osteogenic and adipogenic lineages.
HPL and Hplasma prepared from blood products after their recommended transfusion date can be used as an alternative and effective source for large-scale
expansion of ADMSCs.
J. Phetfong, T. Tawonsawatruk, K. Seenprachawong, A. Srisarin, C. Isarankura-Na-Ayudhya, A. Supokawej. Re-using blood products as an alternative supplement in the optimisation of clinical-grade adipose-derived mesenchymal stem cell culture. Bone Joint Res 2017;6:414-422. DOI: 10.1302/2046-3758.67.BJR-2016-0342.R1.</description><subject>Adipose-Derived Mesenchymal Stem Cells</subject><subject>Blood products</subject><subject>Bone healing</subject><subject>Cell culture</subject><subject>Cell differentiation</subject><subject>Dietary supplements</subject><subject>Fetal calf serum</subject><subject>Fibroblasts</subject><subject>Human Plasma</subject><subject>Mesenchymal Stem Cells</subject><subject>Mesenchyme</subject><subject>Osteogenesis</subject><subject>Phenotypes</subject><subject>Platelet Lysate</subject><subject>Platelets</subject><subject>Senescence</subject><subject>Stem Cell Culture</subject><subject>Stem cells</subject><issn>2046-3758</issn><issn>2046-3758</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpdkV1rFDEUhoMotqz9CxIQwZtZ8zHJTC4UbPGTgrDodcgmZ3ZTMsmYzBT6D_zZZmhdqiGQwHnfw3nPg9BrSraUE_aWkVY2vBP9Vnbby2-7hhEqG8Jbtt3RJ-j8VH_66H-GLkq5IfXIvpWkf47OWN8xIok8R7930CzFxwPeh5QcnnJyi50LNvVGbMIMOZrZ3wIuyzQFGCHO2Ec8HwGnafajL7WcIk4DtsFHb01oDtk4wMb5KRVoHOTqd3iEAtEe70YTcJlhxBZCwHYJ85LhBXo2mFDg4uHdoJ-fPv64-tJcf__89erDdWNbwedGmkEQaXrOemKZopyqToIYQLGuM5LubcsU7J0TXDDlhn0noIPWGOFsjaz4Br2_7zst-xGcrXGyCXrKfjT5Tifj9b-V6I_6kG61EFxVBrXBm4cGOf1aoMy6rmBNYiKkpWiqGOFKkDruBr36T3qTlrrOUDTjlLSt6JWsqnf3KptTKRmG0zCU6BW7XlHqFaWWna7Y9Ypdr9j1jlb_y8eJTu6_kPkfB3StDQ</recordid><startdate>20170701</startdate><enddate>20170701</enddate><creator>Phetfong, J</creator><creator>Tawonsawatruk, T</creator><creator>Seenprachawong, K</creator><creator>Srisarin, A</creator><creator>Isarankura-Na-Ayudhya, C</creator><creator>Supokawej, A</creator><general>British Editorial Society of Bone & Joint Surgery</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>NAPCQ</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170701</creationdate><title>Re-using blood products as an alternative supplement in the optimisation of clinical-grade adipose-derived mesenchymal stem cell culture</title><author>Phetfong, J ; Tawonsawatruk, T ; Seenprachawong, K ; Srisarin, A ; Isarankura-Na-Ayudhya, C ; Supokawej, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-6af506a83280c29131976e5fe9277a61bc429ebdd53529dfb75e7e4aa5dc20693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adipose-Derived Mesenchymal Stem Cells</topic><topic>Blood products</topic><topic>Bone healing</topic><topic>Cell culture</topic><topic>Cell differentiation</topic><topic>Dietary supplements</topic><topic>Fetal calf serum</topic><topic>Fibroblasts</topic><topic>Human Plasma</topic><topic>Mesenchymal Stem Cells</topic><topic>Mesenchyme</topic><topic>Osteogenesis</topic><topic>Phenotypes</topic><topic>Platelet Lysate</topic><topic>Platelets</topic><topic>Senescence</topic><topic>Stem Cell Culture</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Phetfong, J</creatorcontrib><creatorcontrib>Tawonsawatruk, T</creatorcontrib><creatorcontrib>Seenprachawong, K</creatorcontrib><creatorcontrib>Srisarin, A</creatorcontrib><creatorcontrib>Isarankura-Na-Ayudhya, C</creatorcontrib><creatorcontrib>Supokawej, A</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Bone & joint research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Phetfong, J</au><au>Tawonsawatruk, T</au><au>Seenprachawong, K</au><au>Srisarin, A</au><au>Isarankura-Na-Ayudhya, C</au><au>Supokawej, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Re-using blood products as an alternative supplement in the optimisation of clinical-grade adipose-derived mesenchymal stem cell culture</atitle><jtitle>Bone & joint research</jtitle><addtitle>Bone Joint Res</addtitle><date>2017-07-01</date><risdate>2017</risdate><volume>6</volume><issue>7</issue><spage>414</spage><epage>422</epage><pages>414-422</pages><issn>2046-3758</issn><eissn>2046-3758</eissn><abstract>Adipose-derived mesenchymal stem cells (ADMSCs) are a promising strategy for orthopaedic applications, particularly in bone repair.
expansion of ADMSCs is required to obtain sufficient cell numbers. Xenogenic supplements should be avoided in order to minimise the risk of infections and immunological reactions. Human platelet lysate and human plasma may be an excellent material source for ADMSC expansion. In the present study, use of blood products after their recommended transfusion date to prepare human platelet lysate (HPL) and human plasma (Hplasma) was evaluated for
culture expansion and osteogenesis of ADMSCs.
Human ADMSCs were cultured in medium supplemented with HPL, Hplasma and a combination of HPL and Hplasma (HPL+Hplasma). Characteristics of these ADMSCs, including osteogenesis, were evaluated in comparison with those cultured in fetal bovine serum (FBS).
HPL and HPL+Hplasma had a significantly greater growth-promoting effect than FBS, while Hplasma exhibited a similar growth-promoting effect to that of FBS. ADMSCs cultured in HPL and/or Hplasma generated more colony-forming unit fibroblasts (CFU-F) than those cultured in FBS. After long-term culture, ADMSCs cultured in HPL and/or Hplasma showed reduced cellular senescence, retained typical cell phenotypes, and retained differentiation capacities into osteogenic and adipogenic lineages.
HPL and Hplasma prepared from blood products after their recommended transfusion date can be used as an alternative and effective source for large-scale
expansion of ADMSCs.
J. Phetfong, T. Tawonsawatruk, K. Seenprachawong, A. Srisarin, C. Isarankura-Na-Ayudhya, A. Supokawej. Re-using blood products as an alternative supplement in the optimisation of clinical-grade adipose-derived mesenchymal stem cell culture. Bone Joint Res 2017;6:414-422. DOI: 10.1302/2046-3758.67.BJR-2016-0342.R1.</abstract><cop>England</cop><pub>British Editorial Society of Bone & Joint Surgery</pub><pmid>28720606</pmid><doi>10.1302/2046-3758.67.BJR-2016-0342.R1</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Bone & joint research, 2017-07, Vol.6 (7), p.414-422 |
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| subjects | Adipose-Derived Mesenchymal Stem Cells Blood products Bone healing Cell culture Cell differentiation Dietary supplements Fetal calf serum Fibroblasts Human Plasma Mesenchymal Stem Cells Mesenchyme Osteogenesis Phenotypes Platelet Lysate Platelets Senescence Stem Cell Culture Stem cells |
| title | Re-using blood products as an alternative supplement in the optimisation of clinical-grade adipose-derived mesenchymal stem cell culture |
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