Xenogeneic human umbilical cord-derived mesenchymal stem cells reduce mortality in rats with acute respiratory distress syndrome complicated by sepsis

• Published in: Oncotarget 2017-07, Vol.8 (28), p.45626-45642
• Main Authors: Lee, Fan-Yen, Chen, Kuan-Hung, Wallace, Christopher Glenn, Sung, Pei-Hsun, Sheu, Jiunn-Jye, Chung, Sheng-Ying, Chen, Yung-Lung, Lu, Hung-I, Ko, Sheung-Fat, Sun, Cheuk-Kwan, Chiang, Hsin-Ju, Chang, Hsueh-Wen, Lee, Mel S, Yip, Hon-Kan
• Format: Article
• Language: English
• Subjects: Acute Kidney Injury - etiology; Animals; Apoptosis; Biomarkers; Blood Pressure; Cytokines - metabolism; Disease Models, Animal; Flow Cytometry; Heterografts; Humans; Inflammation Mediators - metabolism; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Oxidative Stress; Podocytes - metabolism; Rats; Research Paper; Respiratory Distress Syndrome, Adult - etiology; Respiratory Distress Syndrome, Adult - mortality; Respiratory Distress Syndrome, Adult - physiopathology; Respiratory Distress Syndrome, Adult - therapy; Sepsis - complications; Umbilical Cord - cytology; WT1 Proteins - genetics; WT1 Proteins - metabolism
• ISSN: 1949-2553; 1949-2553
• DOI: 10.18632/oncotarget.17320

This study tested the hypothesis that xenogeneic human umbilical cord-derived mesenchymal stem cell (HUCDMSC) therapy would improve survival rates in rats with acute respiratory distress-syndrome (ARDS, induction by 48 h inhalation of 100% oxygen) and sepsis-syndrome (SS, induction by cecal-ligation and puncture) (ARDS-SS). Adult-male Sprague-Dawley rats were categorized into group 1 (sham-controls), group 2 (ARDS-SS), group 3 [ARDS-SS+HUCDMSC (1.2 ×106 cells administered 1 h after SS-induction)], and group 4 [ARDS-SS+HUCDMSC (1.2 ×106 cells administered 24 h after SS-induction)]. The mortality rate was higher in groups 2 and 4 than in groups 1 and 3 (all p