The structure of the human c‐fes/fps proto‐oncogene

We have determined the complete nucleotide sequence of a human DNA fragment of approximately 13 kbp, which was shown by Southern blot analysis to contain the entire v‐fes/fps cellular homolog. The v‐fes/fps homologous sequences were dispersed over 11 kbp in 18 interspersed segments which were flanke...

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Bibliographic Details
Published in:The EMBO journal 1985-11, Vol.4 (11), p.2897-2903
Main Authors: Roebroek, A.J., Schalken, J.A., Verbeek, J.S., Van den Ouweland, A.M., Onnekink, C., Bloemers, H.P., Van de Ven, W.J.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Composition
Base Sequence
Biological and medical sciences
Chickens
Cosmids
Fundamental and applied biological sciences. Psychology
genes
Genes. Genome
Humans
man
Molecular and cellular biology
Molecular genetics
Nucleic Acid Hybridization
nucleotide sequence
Proto-Oncogene Mas
Proto-Oncogenes
Sequence Homology, Nucleic Acid
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Summary:We have determined the complete nucleotide sequence of a human DNA fragment of approximately 13 kbp, which was shown by Southern blot analysis to contain the entire v‐fes/fps cellular homolog. The v‐fes/fps homologous sequences were dispersed over 11 kbp in 18 interspersed segments which were flanked by splice junctions. Fusion of these segments created a DNA fragment in which coding regions similar to those observed in the viral oncogenes v‐fes of the Gardner‐Arnstein (GA) and Snyder‐Theilen (ST) strains of feline sarcoma virus and v‐fps found in Fujinami sarcoma virus could be identified. A potential initiation site in the first exon was found. About 200 nucleotides downstream of a translational stop codon in the v‐fes/fps homologous region, a poly(A) addition signal was identified. The deduced amino acid sequence has a molecular weight of 93 390 dalton resembling NCP92, the recently described human c‐fes/fps product. The topography of human c‐fes/fps appeared to resemble that of chicken c‐fps.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1985.tb04020.x