Human T lymphocyte clones specific for malaria (Plasmodium falciparum) antigens

Peripheral blood mononuclear cells (PBM) from a patient who had lived in a malarial‐endemic area were cultured in the presence of malarial antigens (a lysate of Plasmodium‐infected erythrocytes). Responding cells were grown in IL‐2‐containing medium and then cloned, and subsequently subcloned, in th...

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Bibliographic Details
Published in:The EMBO journal 1985-12, Vol.4 (13B), p.3819-3822
Main Authors: Sinigaglia, F., Richard, J., Pink, L.
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Antigens, Protozoan - analysis
Antigens, Surface - analysis
Biological and medical sciences
Cell Transformation, Viral
Cells, Cultured
Clone Cells
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Herpesvirus 4, Human - genetics
Human protozoal diseases
Humans
Immunobiology
Infectious diseases
Lymphocyte Activation
Malaria
Malaria - immunology
Medical sciences
Molecular Weight
Organs and cells involved in the immune response
Parasitic diseases
Plasmodium falciparum
Plasmodium falciparum - immunology
Protozoal diseases
T-Lymphocytes - immunology
Tropical medicine
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Summary:Peripheral blood mononuclear cells (PBM) from a patient who had lived in a malarial‐endemic area were cultured in the presence of malarial antigens (a lysate of Plasmodium‐infected erythrocytes). Responding cells were grown in IL‐2‐containing medium and then cloned, and subsequently subcloned, in the presence of phytohemagglutinin and allogeneic irradiated PBM. Ten clones were specific for malarial antigens. They proliferated in response to P. falciparum extract, but not to a lysate of uninfected erythrocytes. The response was HLA‐restricted. All the clones tested responded to lysates of cells infected with parasites of either African or Asian origin. Six clones had the T4+/T8‐ phenotype and four the T4‐/T8+ phenotype. Two of the T4+ clones recognised a parasite antigen of apparent mol. wt. approximately 50 000. All of the clones tested produced gamma‐interferon following antigen stimulation.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1985.tb04153.x