Structural organization of the rat gene for the arginine vasopressin‐neurophysin precursor

The rat arginine vasopressin‐neurophysin precursor gene has been isolated from a genomic library cloned in lambda phage Charon 4A. Restriction mapping and nucleotide sequence analysis demonstrated that the gene is 1.85 kilobase pairs long and contains two intervening sequences located in the protein...

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Bibliographic Details
Published in:The EMBO journal 1983-01, Vol.2 (5), p.763-767
Main Authors: Schmale, H., Heinsohn, S., Richter, D.
Format: Article
Language:English
Subjects:
Animals
Arginine Vasopressin - genetics
Bacteriophage lambda
Base Sequence
Cloning, Molecular
DNA Restriction Enzymes
Neurophysins - genetics
Oxytocin
Protein Precursors - genetics
Rats
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Summary:The rat arginine vasopressin‐neurophysin precursor gene has been isolated from a genomic library cloned in lambda phage Charon 4A. Restriction mapping and nucleotide sequence analysis demonstrated that the gene is 1.85 kilobase pairs long and contains two intervening sequences located in the protein coding region. Exon A encodes a putative signal peptide, the hormone arginine vasopressin and the variable N terminus of the carrier protein neurophysin, exon B encodes the highly conserved middle part of neurophysin and exon C its variable C terminus together with glycoprotein. Thus, the three functional domains of the percursor ‐ arginine, vasopressin, neurophysin, glycoprotein ‐ are encoded on three distinct exons.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1983.tb01497.x