A bovine papilloma virus vector with a dominant resistance marker replicates extrachromosomally in mouse and E. coli cells

We describe the construction of a bovine papilloma virus‐based vector (pCGBPV9) which contains a dominant selectable marker and replicates autonomously in both mouse and Escherichia coli cells. This vector contains the complete bovine papilloma virus genome, a ColE1 replication origin and a dominant...

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Bibliographic Details
Published in:The EMBO journal 1983, Vol.2 (9), p.1487-1492
Main Authors: Matthias, P.D., Bernard, H.U., Scott, A., Brady, G., Hashimoto‐Gotoh, T., Schütz, G.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotechnology
Bovine papillomavirus 1 - genetics
Bovine papillomavirus 1 - physiology
Cattle
Cell Line
Cosmids - genetics
Drug Resistance, Viral - genetics
Escherichia coli - genetics
Escherichia coli - virology
Fundamental and applied biological sciences. Psychology
Genes, Dominant
Genes, Viral
Genetic engineering
Genetic Markers
Genetic technics
Genetic Vectors
Kanamycin Resistance - genetics
Methods. Procedures. Technologies
Mice
Plasmids - genetics
Recombination, Genetic
Transfection
Virus Replication
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Summary:We describe the construction of a bovine papilloma virus‐based vector (pCGBPV9) which contains a dominant selectable marker and replicates autonomously in both mouse and Escherichia coli cells. This vector contains the complete bovine papilloma virus genome, a ColE1 replication origin and a dominant selectable marker conferring resistance to kanamycin in bacteria and G418 in eukaryotic cells. A high number of G418R colonies are obtained after transfer of pCGBPV9 into mouse C127 cells. These G418R colonies contain vector DNA which replicates autonomously at approximately 10‐30 copies per cell. The molecules are in most cases unrearranged and can be rescued into E. coli cells by bacterial transformation.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1983.tb01612.x