Cytoplasmic RNA from normal and malignant human cells shows homology to the DNAs of Epstein‐Barr virus and human adenoviruses

Cytoplasmic RNA prepared from several human cell lines and tissues was hybridised to DNA from Epstein‐Barr virus, human adenovirus types 2, 3 and 12 and human papovaviruses BK and JC. RNA from all the cells, regardless of whether or not they were virally infected, hybridised to specific regions of t...

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Bibliographic Details
Published in:The EMBO journal 1983-01, Vol.2 (10), p.1673-1683
Main Authors: Arrand, J.R., Walsh‐Arrand, J.E., Rymo, L.
Format: Article
Language:English
Subjects:
Adenoviruses, Human - genetics
B-Lymphocytes - analysis
Base Sequence
Burkitt Lymphoma - analysis
Cell Line
DNA Restriction Enzymes
DNA, Viral - genetics
Epstein-Barr virus
Herpesvirus 4, Human - genetics
Humans
Leukemia, Lymphoid - analysis
Nucleic Acid Hybridization
RNA - genetics
RNA, Neoplasm - genetics
Species Specificity
Structure-Activity Relationship
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Description
Summary:Cytoplasmic RNA prepared from several human cell lines and tissues was hybridised to DNA from Epstein‐Barr virus, human adenovirus types 2, 3 and 12 and human papovaviruses BK and JC. RNA from all the cells, regardless of whether or not they were virally infected, hybridised to specific regions of the Epstein‐Barr virus or adenovirus genomes but not to papovavirus DNA. The cellular cross‐hybridising species appear to be repetitive sequences which are conserved in higher eukaryotes. Mismatch estimations indicate a high degree of homology between the viral and host sequences. Detailed analysis of selected regions of viral DNA failed to reveal any primary‐structural peculiarities.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1983.tb01642.x