Expression and Clinical Significance of ILF2 in Gastric Cancer

The aim of this study is to investigate the expression levels and clinical significance of ILF2 in gastric cancer. The mRNA and protein expression levels of ILF2 were, respectively, examined by quantitative real-time PCR (qRT-PCR) and Western blot from 21 paired fresh frozen GC tissues and correspon...

Full description

Saved in:
Bibliographic Details
Published in:Disease markers 2017-01, Vol.2017 (2017), p.1-9
Main Authors: Gui, Qian-le, Shi, Wu-bing, Jiang, Xing-wang, Yin, Zi-huan, Yu, Dong-Feng
Format: Article
Language:English
Subjects:
Aged
Biological markers
Biomarkers
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Cancer
Clinical significance
Disease-Free Survival
Female
Gastric cancer
Gene expression
Genetic aspects
Humans
Immunohistochemistry
Lymph nodes
Male
Medical prognosis
Metastases
Middle Aged
mRNA
Multivariate analysis
Neoplasm Metastasis
Nuclear Factor 45 Protein - genetics
Nuclear Factor 45 Protein - metabolism
Paraffin
Prognosis
Protein expression
Proteins
RNA, Messenger - genetics
RNA, Messenger - metabolism
Statistical methods
Stomach cancer
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
Survival
Tissues
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this study is to investigate the expression levels and clinical significance of ILF2 in gastric cancer. The mRNA and protein expression levels of ILF2 were, respectively, examined by quantitative real-time PCR (qRT-PCR) and Western blot from 21 paired fresh frozen GC tissues and corresponding normal gastric tissues. In order to analyze the expression pattern of ILF2 in GC, 60 paired paraffin-embedded GC slides and corresponding normal gastric slides were detected by immunohistochemistry (IHC) assay. The correlation between ILF2 protein expression levels and clinicopathological parameters, overall survival (OS), disease-free survival (DFS), and clinical prognosis were analyzed by statistical methods. Significantly higher levels of ILF2 were detected in GC tissues compared with normal controls at both mRNA and protein level. High expression of ILF2 was tightly correlated with depth of invasion, lymph node metastasis, pathological stage, and histological differentiation. Log-rank test showed that high expression of ILF2 was positively associated with poor clinical prognosis. Multivariate analysis identified that ILF2 was an independent prognostic factor for OS and DFS. Our findings suggest that ILF2 may be a valuable biomarker and a novel potential prognosis predictor for GC patients.
ISSN:0278-0240
1875-8630
DOI:10.1155/2017/4387081