Loading…

Endurance performance is enhanced by intermittent hyperbaric exposure via up‐regulation of proteins involved in mitochondrial biogenesis in mice

This study was designed to (1) investigate the effects of acute hyperbaric exposure on muscle mRNA expression levels, and (2) clarify the mechanisms by which intermittent hyperbaric exposure improves endurance capacity. Experiment 1: Male mice were subjected to acute 1‐h hyperbaric exposure (1.3 atm...

Full description

Saved in:
Bibliographic Details
Published in:Physiological reports 2017-08, Vol.5 (15), p.e13349-n/a
Main Author: Suzuki, Junichi
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study was designed to (1) investigate the effects of acute hyperbaric exposure on muscle mRNA expression levels, and (2) clarify the mechanisms by which intermittent hyperbaric exposure improves endurance capacity. Experiment 1: Male mice were subjected to acute 1‐h hyperbaric exposure (1.3 atmospheres absolute with 20.9% O2). The expression of peroxisome proliferator‐activated receptor gamma coactivator 1‐alpha (PGC‐1α) mRNA significantly increased in the soleus (7.2‐fold) and red gastrocnemius muscles (Gr, 5.1‐fold) 3 h after hyperbaric exposure. Peroxisome proliferator‐activated receptor alpha (PPARα) mRNA levels significantly increased in the plantaris (PL, 2.9‐fold) and Gr (2.3‐fold) 3 h after hyperbaric exposure. Experiment 2: Mice were subjected to exercise training with (HypTr) and without (Tr) 1‐h hyperbaric exposure for 4 weeks. Increases in maximal exercise capacity were significantly greater in HypTr than in Tr. In PL, activity levels of 3‐hydroxyacyl‐CoA‐dehydrogenase and citrate synthase (CS) were significantly greater in HypTr than in Tr. CS and phosphofructokinase activities both markedly increased in the white gastrocnemius muscle (Gw) in HypTr only. PGC‐1α expression in the nucleus was significantly greater in HypTr than in Tr in PL (4.8‐fold), Gr (3.2‐fold), and Gw (15‐fold). Protein levels of mitochondrial transcription factor A and heat shock protein 70 significantly increased after training with hyperbaric exposure. These results suggest that exercise training with intermittent hyperbaric exposure represents a beneficial strategy for increasing endurance performance by facilitating oxidative and glycolytic capacities and the expression of proteins involved in mitochondrial biogenesis in the hindlimb muscles. Exercise training with intermittent hyperbaric exposure represents a beneficial strategy for increasing endurance performance by facilitating oxidative and glycolytic capacities and the expression of proteins involved in mitochondrial biogenesis in the hindlimb muscles.
ISSN:2051-817X
DOI:10.14814/phy2.13349