Synaptotagmin isoforms confer distinct activation kinetics and dynamics to chromaffin cell granules

• Published in: The Journal of general physiology 2017-08, Vol.149 (8), p.763-780
• Main Authors: Rao, Tejeshwar C, Santana Rodriguez, Zuleirys, Bradberry, Mazdak M, Ranski, Alexandra H, Dahl, Peter J, Schmidtke, Michael W, Jenkins, Paul M, Axelrod, Daniel, Chapman, Edwin R, Giovannucci, David R, Anantharam, Arun
• Format: Article
• Language: English
• Subjects: Activation; Animals; Calcium (intracellular); Calcium channels; Calcium Channels - metabolism; Calcium Signaling; Cattle; Cells; Cells, Cultured; Chromaffin cells; Chromaffin Cells - metabolism; Cytoplasmic Granules - metabolism; Depolarization; Endocrine system; Exocytosis; Female; Granular materials; Granule cells; Isoforms; Kinetics; Nervous system; Neuropeptides; Physiology; Protein Isoforms - genetics; Protein Isoforms - metabolism; Proteins; Spatial discrimination; Specialization; Sympathetic nervous system; Synaptotagmin; Synaptotagmins - genetics; Synaptotagmins - metabolism
• ISSN: 0022-1295; 1540-7748
• DOI: 10.1085/jgp.201711757

Adrenomedullary chromaffin cells respond to sympathetic nervous system activation by secreting a cocktail of potent neuropeptides and hormones into the circulation. The distinct phases of the chromaffin cell secretory response have been attributed to the progressive fusion of distinct populations of dense core granules with different activation kinetics. However, it has been difficult to define what distinguishes these populations at the molecular level. Functional segregation of granule pools may depend on selective sorting of synaptotagmin-1 (Syt-1) and synaptotagmin-7 (Syt-7), which our previous work showed are rarely cosorted to the same granule. Here we assess the consequences of selective sorting of Syt isoforms in chromaffin cells, particularly with respect to granule dynamics and activation kinetics. Upon depolarization of cells expressing fluorescent Syt isoforms using elevated K , we find that Syt-7 granules fuse with faster kinetics than Syt-1 granules, irrespective of stimulation strength. Pharmacological blockade of Ca channels reveals differential dependence of Syt-1 versus Syt-7 granule exocytosis on Ca channel subtypes. Syt-7 granules also show a greater tendency to fuse in clusters than Syt-1 granules, and granules harboring Syt-1 travel a greater distance before fusion than those with Syt-7, suggesting that there is spatial and fusion-site heterogeneity among the two granule populations. However, the greatest functional difference between granule populations is their responsiveness to Ca Upon introduction of Ca into permeabilized cells, Syt-7 granules fuse with fast kinetics and high efficacy, even at low Ca levels (e.g., when cells are weakly stimulated). Conversely, Syt-1 granules require a comparatively larger increase in intracellular Ca for activation. At Ca concentrations above 30 µM, activation kinetics are faster for Syt-1 granules than for Syt-7 granules. Our study provides evidence for functional specialization of chromaffin cell granules via selective expression of Syt isoforms with different Ca sensitivities.